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| Status |
Public on Jan 03, 2024 |
| Title |
Heme-TAMs are resistant to tumoricidal transformation by INFγ |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Inflammatory macrophage activation is a critical component of the antitumor immune response and an emerging therapeutic concept. To evaluate the impact of heme exposure on antitumoral macrophage activity, we grew spheroids of mixed GFP-MC38 and BMDMs that were either untreated or pretreated with heme, INFγ, or heme + INFγ, and characterized them by scRNA-seq.
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| Overall design |
Spheroids of MC38-GFP and MC38-GFP mixed with BMDMs that were stimulated with either IFNy, heme, or heme + IFNy were grown in microwell plates for 9 days. 4500 spheroids per condition were pooled and digested into single cells. Cell Multiplexing Oligos (CMO) labels B301 - B305 (3’ CellPlex Kit Set A) were used to label the 5 samples. The 5 samples were pooled and then directly used for GEM generation targeting a cell recovery rate of 10’000 cells.
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| Contributor(s) |
Vallelian F, Schaer D, Baselgia L, Duerst E |
| Citation(s) |
38060331 |
| |
| Submission date |
Jul 10, 2023 |
| Last update date |
Feb 15, 2024 |
| Contact name |
Florence Vallelian |
| E-mail(s) |
florence.vallelian@usz.ch
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| Organization name |
University Hospital Zürich
|
| Street address |
Rämistrasse 100
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| City |
Zurich |
| ZIP/Postal code |
8091 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE237612 |
Hemorrhage-induced NRF2 signaling in tumor-associated macrophages drives cancer growth, invasion, and immunotherapy resistance |
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| Relations |
| BioProject |
PRJNA993273 |
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