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Series GSE236995 Query DataSets for GSE236995
Status Public on Jan 03, 2024
Title Heme-TAMs prevent cancer cell apoptosis and drive growth, invasiveness, and metastasis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To study the impact of heme-TAMs on the growth, phenotype, and function of cancer cells, we cultured GFP-MC38 cells either alone or mixed with heme-treated macrophages in microwell plates, enabling us to collect large numbers of uniform spheroids for scRNA-seq. We observed that after four days, the size of GFP-MC38 spheroids regressed, and the tumor cells underwent apoptosis and decayed metabolically. In contrast, the presence of heme-TAMs within spheroids prevented apoptosis, supporting the extended growth of metabolically viable spheroids beyond 10 days of culture.
 
Overall design Spheroids of MC38-GFP and MC38-GFP mixed with BMDMs that were stimulated with heme were grown in microwell plates for 4, 8, and 10 days. 9000 spheroids per condition were pooled and digested into single cells. Single-cell suspensions were directly used for GEM generation.
 
Contributor(s) Vallelian F, Schaer D, Baselgia L, Duerst E
Citation(s) 38060331
Submission date Jul 10, 2023
Last update date Feb 15, 2024
Contact name Florence Vallelian
E-mail(s) florence.vallelian@usz.ch
Organization name University Hospital Zürich
Street address Rämistrasse 100
City Zurich
ZIP/Postal code 8091
Country Switzerland
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM7592776 tumor cell spheroids day 4
GSM7592777 tumor cell spheroids day 8
GSM7592778 tumor cell spheroids day 10
This SubSeries is part of SuperSeries:
GSE237612 Hemorrhage-induced NRF2 signaling in tumor-associated macrophages drives cancer growth, invasion, and immunotherapy resistance
Relations
BioProject PRJNA993275

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE236995_RAW.tar 1.2 Gb (http)(custom) TAR (of H5)
GSE236995_TC_MAC_processed.h5ad.gz 1.8 Gb (ftp)(http) H5AD
GSE236995_TC_only_processed.h5ad.gz 1.6 Gb (ftp)(http) H5AD
GSE236995_macrophages_processed.h5ad.gz 92.8 Mb (ftp)(http) H5AD
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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