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Status |
Public on Jan 03, 2024 |
Title |
Heme-TAMs prevent cancer cell apoptosis and drive growth, invasiveness, and metastasis |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To study the impact of heme-TAMs on the growth, phenotype, and function of cancer cells, we cultured GFP-MC38 cells either alone or mixed with heme-treated macrophages in microwell plates, enabling us to collect large numbers of uniform spheroids for scRNA-seq. We observed that after four days, the size of GFP-MC38 spheroids regressed, and the tumor cells underwent apoptosis and decayed metabolically. In contrast, the presence of heme-TAMs within spheroids prevented apoptosis, supporting the extended growth of metabolically viable spheroids beyond 10 days of culture.
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Overall design |
Spheroids of MC38-GFP and MC38-GFP mixed with BMDMs that were stimulated with heme were grown in microwell plates for 4, 8, and 10 days. 9000 spheroids per condition were pooled and digested into single cells. Single-cell suspensions were directly used for GEM generation.
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Contributor(s) |
Vallelian F, Schaer D, Baselgia L, Duerst E |
Citation(s) |
38060331 |
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Submission date |
Jul 10, 2023 |
Last update date |
Feb 15, 2024 |
Contact name |
Florence Vallelian |
E-mail(s) |
florence.vallelian@usz.ch
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Organization name |
University Hospital Zürich
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Street address |
Rämistrasse 100
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City |
Zurich |
ZIP/Postal code |
8091 |
Country |
Switzerland |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE237612 |
Hemorrhage-induced NRF2 signaling in tumor-associated macrophages drives cancer growth, invasion, and immunotherapy resistance |
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Relations |
BioProject |
PRJNA993275 |