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Series GSE236766 Query DataSets for GSE236766
Status Public on Oct 05, 2023
Title A single-cell atlas of pig gastrulation as a resource for comparative embryology
Organism Sus scrofa
Experiment type Expression profiling by high throughput sequencing
Summary The bilaminar disc of early pig embryos closely mirrors that of humans making it a powerful model for studying gastrulation. Given the difficulties obtaining embryos in non-rodents, early cell-fate decisions during mammalian gastrulation remain ill-understood. Here we present a single-cell transcriptomic atlas of pig gastrulation and early organogenesis. We uncover the dynamics of cell fate emergence during pig peri-gastrulation and reveal conserved and species-specific transcriptional programs across different mammals. Combined with investigations in embryos and embryonic stem cells, we elucidate the spatial, molecular, and temporal events during definitive endoderm (DE) formation. We show that early FOXA2+ epiblast progenitors become DE without undergoing epithelial-to-mesenchymal transition, contrasting later emerging FOXA2/TBXT+ anterior primitive streak, which form node/notochord progenitors. We demonstrate that DE fate is driven by hypoblast-derived NODAL signalling, which is extinguished upon DE differentiation. These findings highlight the interplay between temporal and topological signalling during early cell fate decisions during mammalian gastrulation.
Overall design Single-cell RNA sequencing of 62 pig embryos at 8 different time points ranging from the beginning of gastrulation (E11.5) through to the 12 somite stage (E15) using the 10X chromium platform.
Contributor(s) Simpson LA, Strange A, Klisch D, Kraunsoe S, Azami T, Goszczynski D, Le T, Planells B, Holmes N, Sang F, Henson S, Loose M, Nichols J, Alberio R
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Submission date Jul 07, 2023
Last update date Oct 06, 2023
Contact name Ramiro Alberio
Phone +447473159996
Organization name University of Nottingham
Department School of Biosciences
Lab Room Room B234 South Lab
Street address Sutton Bonington Campus
City Sutton Bonington
State/province Leicestershire
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL20983 Illumina NextSeq 500 (Sus scrofa)
Samples (24)
GSM7575170 E11.5-pool 1
GSM7575171 E11.5-pool 2
GSM7575172 E12-pool 1
BioProject PRJNA992403

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Supplementary file Size Download File type/resource
GSE236766_All_samples_metadata.csv.gz 3.1 Mb (ftp)(http) CSV
GSE236766_All_samples_raw_counts.csv.gz 265.6 Mb (ftp)(http) CSV
GSE236766_RAW.tar 1.7 Gb (http)(custom) TAR (of TAR)
Raw data not provided for this record
Processed data provided as supplementary file
Processed data are available on Series record

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