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| Status |
Public on Dec 06, 2023 |
| Title |
Effects of strigolactone analogue dGR24 on the transcriptome of Bacillus subtillis |
| Organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Objective: to compare and contrast the effects of exogenous application of strigolatone analogue rac-dGR24, on gene expression in bacillus subtilis. The experiment compared the effects on three genotypes: wild type and two mutant lines, a ∆rsbQ and RsbQS96A.
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| Overall design |
Two treatments (mock, 10 µM rac-dGR24) were applied to three genotypes (WT, ∆rsbQ, RsbQS96A) in triplicate at an optical density (OD 600) ranging from 1.0 to 1.1. Cultures were harvested at OD ranging from 1.4 to 1.5 and transferred to liquid nitrogen prior to extraction of total RNA.
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| Contributor(s) |
Kamran M, Waters M |
| Citation missing |
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| |
| Submission date |
Jul 07, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Muhammad Kamran |
| E-mail(s) |
pplantscience@gmail.com
|
| Phone |
0472717390
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| Organization name |
The University of Western Australia
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| Department |
School of Molecular sciences
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| Lab |
Waters Lab
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| Street address |
35 Stirling Hwy, Crawley WA
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| City |
Perth |
| State/province |
WA |
| ZIP/Postal code |
6009 |
| Country |
Australia |
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| Platforms (1) |
| GPL30886 |
Illumina NovaSeq 6000 (Bacillus subtilis) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA992383 |
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