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Series GSE23669 Query DataSets for GSE23669
Status Public on Aug 19, 2010
Title Changes in polysome loading as a consequence of RHA downregulation [Agilent]
Platform organism Homo sapiens
Sample organism Chlorocebus aethiops
Experiment type Expression profiling by array
Summary RNA helicase A (RHA) binds its target transcripts at the post-transcriptional control element (PCE) located in the 5’ untranslated region (UTR). This interaction represents an “RNA switch” that regulates protein synthesis. Down regulation of RHA by siRNAs was used to identify transcripts with RHA-dependent translation. Reduced accumulation of RNA in polysomes was monitored with microarrays.
Overall design Cytoplasmic lysates of cells treated with RHA targeted or non-silencing control siRNAs were separated by sucrose density gradient centrifugation. Ribosomal RNA profiles were generated, containing heavy polysomes were collected, and RNA was extracted.
Contributor(s) Boris-Lawrie K, Hernandez JM
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Submission date Aug 17, 2010
Last update date Feb 22, 2018
Contact name Kathleen Boris-Lawrie
Phone (614) 292-1392
Fax (614) 292-6473
Organization name The Ohio State University
Department Department of Veterinary Biosciences
Street address 1925 Coffey Rd
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (3)
GSM580634 Polysome Experiment Rep 1
GSM580635 Polysome Experiment Rep 2
GSM580636 Polysome Experiment Rep 3
This SubSeries is part of SuperSeries:
GSE23689 Identification of RNA helicase A translation target mRNAs at the intersection of RNA precipitation and polysome arrays
BioProject PRJNA133143

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23669_RAW.tar 19.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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