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| Status |
Public on Aug 19, 2010 |
| Title |
Changes in polysome loading as a consequence of RHA downregulation [Agilent] |
| Platform organism |
Homo sapiens |
| Sample organism |
Chlorocebus aethiops |
| Experiment type |
Expression profiling by array
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| Summary |
RNA helicase A (RHA) binds its target transcripts at the post-transcriptional control element (PCE) located in the 5’ untranslated region (UTR). This interaction represents an “RNA switch” that regulates protein synthesis. Down regulation of RHA by siRNAs was used to identify transcripts with RHA-dependent translation. Reduced accumulation of RNA in polysomes was monitored with microarrays.
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| Overall design |
Cytoplasmic lysates of cells treated with RHA targeted or non-silencing control siRNAs were separated by sucrose density gradient centrifugation. Ribosomal RNA profiles were generated, containing heavy polysomes were collected, and RNA was extracted.
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| Contributor(s) |
Boris-Lawrie K, Hernandez JM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 17, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Kathleen Boris-Lawrie |
| E-mail(s) |
Kathleen.Boris-Lawrie@cvm.osu.edu
|
| Phone |
(614) 292-1392
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| Fax |
(614) 292-6473
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| URL |
http://www.vet.ohio-state.edu/KathleenBoris-Lawrie.htm
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| Organization name |
The Ohio State University
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| Department |
Department of Veterinary Biosciences
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| Street address |
1925 Coffey Rd
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE23689 |
Identification of RNA helicase A translation target mRNAs at the intersection of RNA precipitation and polysome arrays |
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| Relations |
| BioProject |
PRJNA133143 |
