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| Status |
Public on Jan 03, 2024 |
| Title |
Mixed cell-type spheroids endorse the generation and maintenance of heme-TAMs in an experimental tumor microenvironment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To more accurately mimic the tumor microenvironment, we established a three-dimensional culture of MC38 cancer cells mixed with heme-treated BMDMs in microwell plates. This resulted in tumor spheroids with uniformly interspersed macrophages. We profiled the transcriptome of macrophages within these spheroids using scRNA-seq 24 hours after spheroid formation. To test the robustness of the heme effects in this model, we conducted a multiplexed experiment evaluating heme alone and in combination with the classical M1-polarization stimuli INFγ and LPS.
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| Overall design |
We profiled the transcriptome of macrophages within BMDM + MC38-GFP spheroids using scRNA-seq 24 hours after spheroid formation. To test the robustness of the heme effects in this model, we conducted a multiplexed experiment evaluating heme alone and in combination with the classical M1-polarization stimuli INFγ and LPS.
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| Contributor(s) |
Vallelian F, Schaer D, Baselgia L, Duerst E |
| Citation(s) |
38060331 |
| |
| Submission date |
Jul 05, 2023 |
| Last update date |
Feb 15, 2024 |
| Contact name |
Florence Vallelian |
| E-mail(s) |
florence.vallelian@usz.ch
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| Organization name |
University Hospital Zürich
|
| Street address |
Rämistrasse 100
|
| City |
Zurich |
| ZIP/Postal code |
8091 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (5)
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| GSM7558128 |
heme-treated BMDM + MC38-GFP spheroids (24h) |
| GSM7558129 |
heme/interferon-treated BMDM + MC38-GFP spheroids (24h) |
| GSM7558130 |
heme/LPS-treated BMDM + MC38-GFP spheroids (24h) |
| GSM7558132 |
interferon-treated BMDM + MC38-GFP spheroids (24h) |
| GSM7558133 |
LPS-treated BMDM + MC38-GFP spheroids (24h) |
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| This SubSeries is part of SuperSeries: |
| GSE237612 |
Hemorrhage-induced NRF2 signaling in tumor-associated macrophages drives cancer growth, invasion, and immunotherapy resistance |
|
| Relations |
| BioProject |
PRJNA991594 |
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