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Series GSE236469 Query DataSets for GSE236469
Status Public on Jan 13, 2024
Title Gene expression profile of CD11b+ Gr1+ Myeloid derived suppressor cells isolated from tumor microenviroment and bone marrow oat different stages of tumor bearing animals and control animals.
Organism Mus musculus
Experiment type Expression profiling by array
Summary To understand changes in gene expression profile of myeloid derived suppressor cells in tumor microenvironment and bone marrow with the shift of immune paradigm in response to progressive tumor developments.
 
Overall design CD11b+ Gr1+ Myeloid derived suppressor cells were isolated using FACS from ascites and bone marrow from S-180 tumor bearing animals of 4 stages of tumor as 3day post tumor innoculation, 7day post tumor innoculation, 14day post tumor innoclation, 21day pos tumor innoculation which is early to late phase tumor transition and the normal counter part also isolated to compare the gene expression profile difference between control and tumor microenvironment derived MDSC. Whole cell mRNA were isolated using ambion RNA isolateion mini kit. Each group of sample represents two biological replicates (Control1, Control2), each replicate contains pulled RNA from 12 animals. 5 groups of samples as control, 3day, 7day, 14day, 21day containg one replicate in both ascites and bone marrow in total 20 samples were analysed.
Web link https://academic.oup.com/jleukbio/advance-article-abstract/doi/10.1093/jleuko/qiae013/7571483?redirectedFrom=fulltext&login=false
 
Contributor(s) Mahanti K, Saha J, Sarkar D, Pramanik A, Bhattacharyya S
Citation(s) 38236200
Submission date Jul 05, 2023
Last update date Jan 20, 2024
Contact name Sankar Bhattacharyya
E-mail(s) sankar-bhattacharyya@skbu.ac.in
Phone 09674014065
Organization name Sidho Kanho Birsha University
Department Zoology
Lab Immunobiology and Translational Medicine
Street address Ranchi Road Sainik School
City Purulia
State/province West Bengal
ZIP/Postal code 723104
Country India
 
Platforms (1)
GPL32980 Agilent Mouse Gene Expression 8X60k (AMADID: 74809)
Samples (20)
GSM7548307 Bone marrow, MDSC, 3day1
GSM7548308 Bone marrow, MDSC, 3day2
GSM7548309 Bone marrow, MDSC, control1
Relations
BioProject PRJNA991435

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE236469_Ascites_processed_data.xlsx 6.3 Mb (ftp)(http) XLSX
GSE236469_Bone_marrow_processed_data.xlsx 3.6 Mb (ftp)(http) XLSX
GSE236469_Normalized_signal_intensity.xlsx 12.9 Mb (ftp)(http) XLSX
GSE236469_RAW.tar 62.3 Mb (http)(custom) TAR (of TXT)
Processed data are available on Series record

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