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Status |
Public on Mar 15, 2024 |
Title |
Atf4 promotes erythroid differentiation by regulating ribosome biogenesis via RPS19BP1 |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To elucidate the mechanisms underlying the role of Atf4 deficiency in erythropoiesis, we performed 10X Genomics single-cell RNA sequencing on Lin-cKit+ cells from Atf4 hematopoietic cell-specific conditional knockout mice (KO) and Atf4 floxed mice (WT) at steady state (referred to KO_BM and WT_BM) and after secondary transplantation (referred to cKO_t and WT_t). Furthermore, we also sorted CMP cells from Atf4 hematopoietic cell-specific conditional knockout mice (KO) and ATF4 floxed mice (WT) for 10X Genomics scRNA-seq.
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Overall design |
We performed 10X Genomics scRNA-seq of fluorescence-activated cell sorter (FACS)-sorted bone marrow (BM) Lin-cKit+ cells from Atf4 floxed mice (WT) and Mx1cre; Atf4fl/fl (KO) mice at 4 weeks post-deletion. We also enriched Lin-cKit+ cells from the BM of secondary recipient mice for an additional scRNA-seq analysis. In total, we captured 33,237 cells, of which 29,026 passed quality control. To further determine the transcriptional profiles in CMP cells, we performed 10x Genomics scRNA-seq of FACS-sorted CMP cells from WT and KO mice at 4 weeks post-deletion. And 19,706 of 21,914 sequenced CMP cells were kept after quality control.
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Contributor(s) |
Cheng T, Cheng H, Zheng Z, Yang S |
Citation missing |
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Submission date |
Jun 26, 2023 |
Last update date |
Mar 16, 2024 |
Contact name |
chao tang |
E-mail(s) |
tangchao@ihcams.ac.cn
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Organization name |
Institute of Hematology and Blood Diseases Hospital
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Street address |
288 Nanjing Road
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City |
Tianjin |
State/province |
Tianjin |
ZIP/Postal code |
300000 |
Country |
China |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA987998 |