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Series GSE235166 Query DataSets for GSE235166
Status Public on Jun 20, 2023
Title Assessment of the molecular mechanism associated with osteogenic and angiogenic differentiation induced by FeMnSi scaffold
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate the molecular mechanism underlying osteogenic and angiogenic differentiation induced by the De-HA scaffold, we conducted an in-depth gene microarray analysis on day 3 of MC3T3-E1 culture. We found that De-HA could enhance the transcription of osteogenic and angiogenic genes by activating FAK and downstream MAPK/ERK signaling to induce MC3T3-E1 differentiation. More importantly, our findings indicated that EGFR, a kind of transmembrane glycoprotein, played a key role in the osteogenic differentiation of MC3T3-E1, suggesting that the activation of the FAK/MAPK/ERK signaling cascade might be driven by EGFR.
 
Overall design The scaffold samples were segregated into two groups: the first comprised of the original FeMnSi scaffold, while the second consisted of a De-HA scaffold that had been dealloyed and coated with bioactive materials. MC3T3-E1 were seeded on each scaffold at a density of 1 × 105 per/well in 24-well plates and then cultured for 3 days. An extensive gene microarray analysis was conducted on day 3 of MC3T3-E1 culture.
 
Contributor(s) Yuan B, Chen Z
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Submission date Jun 16, 2023
Last update date Jun 23, 2023
Contact name Bo Yuan
E-mail(s) scuyb@scu.edu.cn
Organization name Sichuan University
Street address Jiuyanqiao Wangjiang Road
City Chengdu
ZIP/Postal code 610064
Country China
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (6)
GSM7496229 De-HA-1
GSM7496230 De-HA-2
GSM7496231 De-HA-3
Relations
BioProject PRJNA984698

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE235166_RAW.tar 12.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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