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Series GSE235102 Query DataSets for GSE235102
Status Public on Dec 06, 2023
Title Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Adult muscle stem cell (MuSC) behavior has been predominantly studied in the context of regeneration, a condition under which MuSCs exit quiescence and replicate upon activation before differentiating and fusing into myocytes. We use single-cell RNA-seq (scRNA-seq) to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus.
 
Overall design MuSCs from plantaris muscles of doxyxycline treated Pax7rtTA; TRE-H2B-GFP mice, subjected to either sham surgery or 5-days of a growth stimulus, were isolated by Fluorescence-activated cell sorting (FACS) according to the prescence of GFP signal and analyzed using scRNA-seq.
 
Contributor(s) Ismaeel A, Wen Y, Peterson C, McCarthy JJ
Citation(s) 38066665
Submission date Jun 15, 2023
Last update date Dec 11, 2023
Contact name Ahmed Ismaeel
E-mail(s) ahmed.ismaeel@uky.edu
Organization name University of Kentucky
Department Physiology
Lab Center for Muscle Biology
Street address 900 S Limestone St
City Lexington
State/province Kentucky
ZIP/Postal code 40536
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM7495019 SHAM, replicate 1, scRNA-seq
GSM7495020 OVERLOAD, replicate 1, scRNA-seq
Relations
BioProject PRJNA984339

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE235102_RAW.tar 53.5 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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