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Series GSE235102 Query DataSets for GSE235102
Status Public on Dec 06, 2023
Title Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Adult muscle stem cell (MuSC) behavior has been predominantly studied in the context of regeneration, a condition under which MuSCs exit quiescence and replicate upon activation before differentiating and fusing into myocytes. We use single-cell RNA-seq (scRNA-seq) to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus.
Overall design MuSCs from plantaris muscles of doxyxycline treated Pax7rtTA; TRE-H2B-GFP mice, subjected to either sham surgery or 5-days of a growth stimulus, were isolated by Fluorescence-activated cell sorting (FACS) according to the prescence of GFP signal and analyzed using scRNA-seq.
Contributor(s) Ismaeel A, Wen Y, Peterson C, McCarthy JJ
Citation(s) 38066665
Submission date Jun 15, 2023
Last update date Dec 11, 2023
Contact name Ahmed Ismaeel
Organization name University of Kentucky
Department Physiology
Lab Center for Muscle Biology
Street address 900 S Limestone St
City Lexington
State/province Kentucky
ZIP/Postal code 40536
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM7495019 SHAM, replicate 1, scRNA-seq
GSM7495020 OVERLOAD, replicate 1, scRNA-seq
BioProject PRJNA984339

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE235102_RAW.tar 53.5 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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