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| Status |
Public on Jan 01, 2024 |
| Title |
CD45+ single cell transcriptomes in the MC38 tumor microenvironment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Colorectal cancer (CRC) continues to increase globally, thus identification of mechanisms that prevent cancer are sorely needed. The microbiota has emerged as a key driver of CRC pathogenesis; however, little is known regarding how specific microbes might prevent CRC. Using transplantation of the microbiota from individuals with or without CRC into germfree mice, we identified that the microbiota harbored by non-diseased individuals can reduce tumor formation and Bacteroides uniformis was a potentially protective member of the microbiota. Single cell sequencing of CD45+ immune cells within the MC38 tumor shows a change in T and NK cell populations with B. uniformis treatment. T cell deficient mice were still protected from CRC in response to B. uniformis treatment, while NK cell depletion eradicated microbe-mediated protection. B. uniformis is reduced in individuals with CRC and thus our data identify B. uniformis as a microbe enriched in healthy individuals that can reduce tumor formation through enhanced NK cell activity.
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| Overall design |
Sorted CD45+ cells from the MC38 tumor microenvironment from mice with or without B. uniformis oral gavage supplementation. Cells from 10 male or 10 female mice were pooled for each sample.
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| Contributor(s) |
Stephens WZ, Weis AW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 13, 2023 |
| Last update date |
Jan 01, 2024 |
| Contact name |
W. Zac Stephens |
| Organization name |
University of Utah
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| Department |
Pathology
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| Lab |
Round Lab
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| Street address |
15 N. Medical Drive, EEJMRB 4208, EEJMRB 4208
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| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84121-3808 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| GSM7476125 |
18913X1, MC38 Tumor-infiltrating CD45+ cells, PBS gavage, female |
| GSM7476126 |
18913X2, MC38 Tumor-infiltrating CD45+ cells, PBS gavage, male |
| GSM7476127 |
18913X3, MC38 Tumor-infiltrating CD45+ cells, B. uniformis gavage, female |
| GSM7476128 |
18913X4, MC38 Tumor-infiltrating CD45+ cells, B. uniformis gavage, male |
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| Relations |
| BioProject |
PRJNA983400 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234875_18931_MEX.tar.gz |
108.7 Mb |
(ftp)(http) |
TAR |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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