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| Status |
Public on Sep 13, 2023 |
| Title |
Identifying early, intermediate, and late responses to GSK3a inhibition in Jurkat T-ALL cells in the presence or absence of an asparagine depletion |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Amino acid availability is a crucial factor for survivability of cancer cells. Leukemia cells have been shown to resist asparagine depletion by utilizing GSK3-dependent proteasomal degradation, termed Wnt-dependent stabilization of proteins (Wnt/STOP), to replenish their amino acid pool. Inhibition of GSK3a halts the sourcing of amino acids, which subsequently leads to cancer cell vulnerability towards asparaginase therapy. Leveraging RNA sequencing we show that GSK3a inhibition leads to temporally dynamic downregulation of distinct ribosomal proteins upon amino acid starvation.
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| Overall design |
To investigate the early, intermediate, and late responses to GSK3a inhibition in Jurkat T-ALL cells in the presence or absence of an asparagine depletion, a robust GSK3a knockdown was induced in Jurkat T-ALL cells using shRNA.
Subsequently the cells were treated with vehicle or asparaginase, and harvested at 8, 16, 32, and 56 hours of treatment.
Comparative gene expression analysis was performed compairing: timepoints, genotypes and treatment conditions.
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| Contributor(s) |
Loxha L, Ibrahim NK, Stasche AS, Cinar B, Niessen J, Schreek S, Fehlhaber B, Forster M, Stanulla M, Hinze L |
| Citation(s) |
37686063 |
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| Submission date |
Jun 13, 2023 |
| Last update date |
Sep 13, 2023 |
| Contact name |
Laura Hinze |
| E-mail(s) |
hinze.laura@mh-hannover.de
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| Organization name |
Hannover Medical School
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| Street address |
Carl-Neuberg-Straße 1
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| City |
Hannover |
| State/province |
Niedersachsen |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| GSM7474968 |
shGSK3a, 32h, asparaginase |
| GSM7474969 |
shGSK3a, 32h, vehicle |
| GSM7474970 |
shGSK3a, 56h, asparaginase |
| GSM7474971 |
shGSK3a, 56h, vehicle |
| GSM7474972 |
shGSK3a, 8h, asparaginase |
| GSM7474973 |
shGSK3a, 8h, vehicle |
| GSM7474974 |
shLuc, 0h |
| GSM7474975 |
shLuc, 16h, asparaginase |
| GSM7474976 |
shLuc, 16h, vehicle |
| GSM7474977 |
shLuc, 32h, asparaginase |
| GSM7474978 |
shLuc, 32h, vehicle |
| GSM7474979 |
shLuc, 56h, asparaginase |
| GSM7474980 |
shLuc, 56h, vehicle |
| GSM7474981 |
shLuc, 8h, asparaginase |
| GSM7474982 |
shLuc, 8h, vehicle |
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| This SubSeries is part of SuperSeries: |
| GSE234803 |
Amino acid availability is a crucial factor for survivability of cancer cells |
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| Relations |
| BioProject |
PRJNA983259 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234800_shGSK3a_asparaginase_timerseries.tsv.gz |
29.1 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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