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Series GSE234252 Query DataSets for GSE234252
Status Public on Jul 05, 2023
Title Human prostate organoid generation and the identification of prostate development drivers using inductive rodent tissues
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Mechanistic links between development and disease can be exploited to provide clues to the cellular signalling pathways that might become re-activated during adult pathogenesis such as those affecting the prostate. However, the mechanisms underpinning prostate development require further characterisation. Previously, our group developed novel methods to produce prostate organoids using induced pluripotent stem cells (iPSCs). Here, we show that the iPSCs can be fully differentiated into prostate organoids using neonatal rodent seminal vesicle mesenchyme (SVM) in vitro. Additionally, we elucidated molecular drivers of prostate induction through RNA-sequencing analyses of the rat urogenital sinus (the embryonic precursor of the prostate gland) as well as the neonatal seminal vesicles. We identified candidate drivers evident in the inductive mesenchyme and epithelium involved with prostate specification. Our top candidates included Spx, Trib3, Snai1, Snai2, Nrg2, and Lrp4. This work lays the foundations to further interrogate the reactivation of developmental genes in adulthood, leading to prostate disease.
Overall design Tissues from rats known to induce prostate development were isolated. RNA-sequencing of the tissues identified candidate drivers of prostate development. Co-culture of seminal vesicles with human iPSCs in vitro generated prostate organoids.
Contributor(s) Singh P, Lanman NA, Kendall HL, Wilson L, Long R, Franco OE, Buskin A, Miles CG, Hayward SW, Heer R, Robson CN
Citation(s) 37376888
Submission date Jun 06, 2023
Last update date Sep 12, 2023
Contact name Nadia Marie Atallah
Organization name Purdue University
Department Center for Cancer Research
Street address 201 S. University St
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (12)
GSM7458233 SVE rep 1
GSM7458234 SVE rep2
GSM7458235 SVE rep3
BioProject PRJNA980629

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Supplementary file Size Download File type/resource
GSE234252_Mergedcounts.csv.gz 517.7 Kb (ftp)(http) CSV
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