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| Status |
Public on Mar 27, 2024 |
| Title |
Intestinal epithelial adaptations to vertical sleeve gastrectomy defined at single-cell resolution |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The gut plays a key role in regulating metabolic health. Dietary factors disrupt intestinal physiology and contribute to obesity and diabetes, whereas bariatric procedures such as vertical sleeve gastrectomy (VSG) cause gut adaptations that induce robust metabolic improvements. However, our understanding of these adaptations at the cellular and molecular levels remains limited. In a validated murine model, we leverage single-cell transcriptomics to determine how VSG impacts different cell lineages of the small intestinal epithelium. We define cell type-specific genes and pathways that VSG rescues from high-fat diet perturbation and characterize additional rescue-independent changes brought about by VSG. We show that Paneth cells have increased expression of the gut peptide Reg3g after VSG. We also find that VSG restores pathways pertaining to mitochondrial respiration and cellular metabolism, especially within crypt-based cells. Overall, our study provides unprecedented molecular resolution of VSG’s therapeutic effects on the gut epithelium.
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| Overall design |
Single-cell RNA-sequencing of small intestinal epithelial crypts and villi from three groups of adult male C57BL/6J mice after a 16-week dietary intervention and 5 weeks after surgery: (1) high-fat diet VSG mice, (2) high-fat diet sham mice, and (3) low-fat diet sham mice.
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| Contributor(s) |
Sethupathy P, Koch-Laskowski K |
| Citation(s) |
38309446 |
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| Submission date |
Jun 05, 2023 |
| Last update date |
Mar 28, 2024 |
| Contact name |
Kieran Koch-Laskowski |
| E-mail(s) |
klk246@cornell.edu
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| Organization name |
Cornell University
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| Department |
Biomedical Sciences
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| Lab |
Sethupathy Lab
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| Street address |
618 Tower Road, VRT #T7006
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA980255 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234189_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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