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Series GSE234189 Query DataSets for GSE234189
Status Public on Mar 27, 2024
Title Intestinal epithelial adaptations to vertical sleeve gastrectomy defined at single-cell resolution
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The gut plays a key role in regulating metabolic health. Dietary factors disrupt intestinal physiology and contribute to obesity and diabetes, whereas bariatric procedures such as vertical sleeve gastrectomy (VSG) cause gut adaptations that induce robust metabolic improvements. However, our understanding of these adaptations at the cellular and molecular levels remains limited. In a validated murine model, we leverage single-cell transcriptomics to determine how VSG impacts different cell lineages of the small intestinal epithelium. We define cell type-specific genes and pathways that VSG rescues from high-fat diet perturbation and characterize additional rescue-independent changes brought about by VSG. We show that Paneth cells have increased expression of the gut peptide Reg3g after VSG. We also find that VSG restores pathways pertaining to mitochondrial respiration and cellular metabolism, especially within crypt-based cells. Overall, our study provides unprecedented molecular resolution of VSG’s therapeutic effects on the gut epithelium.
Overall design Single-cell RNA-sequencing of small intestinal epithelial crypts and villi from three groups of adult male C57BL/6J mice after a 16-week dietary intervention and 5 weeks after surgery: (1) high-fat diet VSG mice, (2) high-fat diet sham mice, and (3) low-fat diet sham mice.
Contributor(s) Sethupathy P, Koch-Laskowski K
Citation(s) 38309446
Submission date Jun 05, 2023
Last update date Mar 28, 2024
Contact name Kieran Koch-Laskowski
Organization name Cornell University
Department Biomedical Sciences
Lab Sethupathy Lab
Street address 618 Tower Road, VRT #T7006
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM7453704 Villi, High-fat diet, Sham
GSM7453705 Crypts, High-fat diet, Sham
GSM7453706 Villi, High-fat diet, Vertical sleeve gastrectomy
BioProject PRJNA980255

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234189_RAW.tar 1.4 Gb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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