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Series GSE233743 Query DataSets for GSE233743
Status Public on May 30, 2023
Title Comparison of the therapeutic effects between stem cells and exosomes in primary ovarian insufficiency: As promising as cells but different persistency and dosage.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Primary ovarian insufficiency (POI) refers to the loss of ovarian function under the age of 40 and results in amenorrhea and infertility. Our previous studies have shown that transplantation of mesenchymal stem cells (MSCs) and MSC-derived exosomes in chemotherapy-induced POI mouse ovaries can reverse the POI and eventually achieve pregnancy. Based on our recent studies, MSC-derived exosomes have almost equal therapeutic potentials as transplanted MSCs. However, it is still unclear whether exosomes can completely replace MSCs in POI treatment. In this study, we induced POI in C57/BL6 mice by chemotherapy (CXT) using a standard protocol. We compared the RNA expression pattern in ovarian tissue after MSC or exosome treatment. our data suggest that a minimal 10-fold increase, and ideally 100-fold increase, in the exosome concentration is required to generate more analogous results to MSC treatment.
Overall design To find difference between exosome treatment and MSC treatment (10,000 cells), we injected same amount of exosome particle produced from 10,000 cells(1-fold), higher amounts of exosome particles (10-fold and 100-fold) into the POI model and analyzed ovarian follicles. Next, we compared the RNA expression pattern in ovarian tissue. Expriment 1 (Exp1) is MSC treated set, experiment 2 (Exp2) is UC-exosome treated set, experiment 3(Exp3) is BM-exosome treated set.
Web link
Contributor(s) Park H, Al-Hendy A
Citation(s) 37340468
Submission date May 30, 2023
Last update date Aug 29, 2023
Contact name Hang-soo Park
Phone 3126149339
Organization name University of Chicago
Street address 5821 S Drexel Ave
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (38)
GSM7433778 Exp1 Control mouse ovary #1
GSM7433779 Exp1 Control mouse ovary #2
GSM7433780 Exp1 Control mouse ovary #3
BioProject PRJNA977686

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE233743_Exp1_D.vs.T1_deg_down_fdr_0.05_fc_1.5.csv.gz 116.7 Kb (ftp)(http) CSV
GSE233743_Exp1_D.vs.T1_deg_up_fdr_0.05_fc_1.5.csv.gz 96.8 Kb (ftp)(http) CSV
GSE233743_Exp1_D.vs.T1_top_hits.csv.gz 1.0 Mb (ftp)(http) CSV
GSE233743_Exp1_D.vs.T2_deg_down_fdr_0.05_fc_1.5.csv.gz 7.4 Kb (ftp)(http) CSV
GSE233743_Exp1_D.vs.T2_deg_up_fdr_0.05_fc_1.5.csv.gz 2.6 Kb (ftp)(http) CSV
GSE233743_Exp1_D.vs.T2_top_hits.csv.gz 1009.8 Kb (ftp)(http) CSV
GSE233743_Exp1_H.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz 98.4 Kb (ftp)(http) CSV
GSE233743_Exp1_H.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz 126.0 Kb (ftp)(http) CSV
GSE233743_Exp1_H.vs.D_gsea_H.csv.gz 2.1 Kb (ftp)(http) CSV
GSE233743_Exp1_H.vs.D_top_hits.csv.gz 1.0 Mb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz 97.3 Kb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz 116.2 Kb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.D_gsea_H.csv.gz 3.1 Kb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.D_top_hits.csv.gz 1.0 Mb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.T2_deg_down_fdr_0.05_fc_1.5.csv.gz 25.6 Kb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.T2_deg_up_fdr_0.05_fc_1.5.csv.gz 22.0 Kb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.T2_gsea_H.csv.gz 3.8 Kb (ftp)(http) CSV
GSE233743_Exp1_T1.vs.T2_top_hits.csv.gz 1013.3 Kb (ftp)(http) CSV
GSE233743_Exp1_T2.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz 2.6 Kb (ftp)(http) CSV
GSE233743_Exp1_T2.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz 7.4 Kb (ftp)(http) CSV
GSE233743_Exp1_T2.vs.D_gsea_H.csv.gz 2.7 Kb (ftp)(http) CSV
GSE233743_Exp1_T2.vs.D_top_hits.csv.gz 1009.5 Kb (ftp)(http) CSV
GSE233743_Exp1_counts.pc.salmon.limma.voom.rds.gz 1.7 Mb (ftp)(http) RDS
GSE233743_Exp1_fullheatmap_Exp2.tiff.gz 27.4 Kb (ftp)(http) TIFF
GSE233743_Exp1_fullheatmap_onlydiffpairs_Exp2.tiff.gz 28.1 Kb (ftp)(http) TIFF
GSE233743_Exp1_gene_counts_salmon.csv.gz 688.0 Kb (ftp)(http) CSV
GSE233743_Exp1_log2_cpm_filtered_norm.txt.gz 1.2 Mb (ftp)(http) TXT
GSE233743_Exp1_process_count_data_salmon_834_exp2.html.gz 5.5 Mb (ftp)(http) HTML
GSE233743_Exp1_sample_list.docx.gz 10.8 Kb (ftp)(http) DOCX
GSE233743_Exp1_vplot_D.vs.T1.tiff.gz 33.3 Kb (ftp)(http) TIFF
GSE233743_Exp1_vplot_D.vs.T2.tiff.gz 28.6 Kb (ftp)(http) TIFF
GSE233743_Exp1_vplot_H.vs.D.tiff.gz 29.4 Kb (ftp)(http) TIFF
GSE233743_Exp1_vplot_T1.vs.D.tiff.gz 33.4 Kb (ftp)(http) TIFF
GSE233743_Exp1_vplot_T1.vs.T2.tiff.gz 28.6 Kb (ftp)(http) TIFF
GSE233743_Exp1_vplot_T2.vs.D.tiff.gz 28.4 Kb (ftp)(http) TIFF
GSE233743_Exp2_H.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz 17.2 Kb (ftp)(http) CSV
GSE233743_Exp2_H.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz 36.4 Kb (ftp)(http) CSV
GSE233743_Exp2_H.vs.D_gsea_H.csv.gz 3.3 Kb (ftp)(http) CSV
GSE233743_Exp2_H.vs.D_top_hits.csv.gz 1.0 Mb (ftp)(http) CSV
GSE233743_Exp2_T1.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz 672 b (ftp)(http) CSV
GSE233743_Exp2_T1.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz 145 b (ftp)(http) CSV
GSE233743_Exp2_T1.vs.D_gsea_H.csv.gz 3.4 Kb (ftp)(http) CSV
GSE233743_Exp2_T1.vs.D_top_hits.csv.gz 1014.2 Kb (ftp)(http) CSV
GSE233743_Exp2_T2.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz 4.0 Kb (ftp)(http) CSV
GSE233743_Exp2_T2.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz 788 b (ftp)(http) CSV
GSE233743_Exp2_T2.vs.D_gsea_H.csv.gz 2.2 Kb (ftp)(http) CSV
GSE233743_Exp2_T2.vs.D_top_hits.csv.gz 1014.4 Kb (ftp)(http) CSV
GSE233743_Exp2_T3.vs.D_deg_down_fdr_0.05_fc_1.5.csv.gz 133.5 Kb (ftp)(http) CSV
GSE233743_Exp2_T3.vs.D_deg_up_fdr_0.05_fc_1.5.csv.gz 146.6 Kb (ftp)(http) CSV
GSE233743_Exp2_T3.vs.D_gsea_H.csv.gz 3.9 Kb (ftp)(http) CSV
GSE233743_Exp2_T3.vs.D_top_hits.csv.gz 1.0 Mb (ftp)(http) CSV
GSE233743_Exp2_counts.pc.salmon.limma.voom.rds.gz 2.1 Mb (ftp)(http) RDS
GSE233743_Exp2_fullheatmap_Exp1.tiff.gz 26.0 Kb (ftp)(http) TIFF
GSE233743_Exp2_fullheatmap_onlydiffpairs_Exp1.tiff.gz 26.4 Kb (ftp)(http) TIFF
GSE233743_Exp2_gene_counts_salmon.csv.gz 915.0 Kb (ftp)(http) CSV
GSE233743_Exp2_log2_cpm_filtered_norm.txt.gz 1.8 Mb (ftp)(http) TXT
GSE233743_Exp2_process_count_data_salmon_834_exp1.html.gz 5.4 Mb (ftp)(http) HTML
GSE233743_Exp2_sample_list.docx.gz 11.1 Kb (ftp)(http) DOCX
GSE233743_Exp2_vplot_H.vs.D.tiff.gz 29.7 Kb (ftp)(http) TIFF
GSE233743_Exp2_vplot_T1.vs.D.tiff.gz 27.3 Kb (ftp)(http) TIFF
GSE233743_Exp2_vplot_T2.vs.D.tiff.gz 27.5 Kb (ftp)(http) TIFF
GSE233743_Exp2_vplot_T3.vs.D.tiff.gz 30.9 Kb (ftp)(http) TIFF
GSE233743_Exp3_Disease.vs.Control_HALLMARK_E2F_TARGETS_gsea_H.csv.gz 2.8 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_EPITHELIAL_MESENCHYMAL_TRANSITION_gsea_H.csv.gz 1.6 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_MYC_TARGETS_V1_gsea_H.csv.gz 2.3 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_TNFA_SIGNALING_VIA_NFKB_gsea_H.csv.gz 2.3 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_HALLMARK_UV_RESPONSE_DN_gsea_H.csv.gz 1.5 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_deg_down_fdr_0.05_fc_1.5.csv.gz 13.6 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_deg_up_fdr_0.05_fc_1.5.csv.gz 13.8 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_gsea_H.csv.gz 3.4 Kb (ftp)(http) CSV
GSE233743_Exp3_Disease.vs.Control_top_hits.csv.gz 960.9 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt1.vs.Disease_deg_down_fdr_0.05_fc_1.5.csv.gz 156 b (ftp)(http) CSV
GSE233743_Exp3_Trt1.vs.Disease_deg_up_fdr_0.05_fc_1.5.csv.gz 293 b (ftp)(http) CSV
GSE233743_Exp3_Trt1.vs.Disease_top_hits.csv.gz 952.0 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt2.vs.Disease_deg_down_fdr_0.05_fc_1.5.csv.gz 156 b (ftp)(http) CSV
GSE233743_Exp3_Trt2.vs.Disease_deg_up_fdr_0.05_fc_1.5.csv.gz 295 b (ftp)(http) CSV
GSE233743_Exp3_Trt2.vs.Disease_top_hits.csv.gz 932.4 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_DNA_REPAIR_gsea_H.csv.gz 722 b (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_MITOTIC_SPINDLE_gsea_H.csv.gz 1.7 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_MYC_TARGETS_V1_gsea_H.csv.gz 3.0 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_OXIDATIVE_PHOSPHORYLATION_gsea_H.csv.gz 2.5 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_HALLMARK_REACTIVE_OXYGEN_SPECIES_PATHWAY_gsea_H.csv.gz 834 b (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_deg_down_fdr_0.05_fc_1.5.csv.gz 103.2 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_deg_up_fdr_0.05_fc_1.5.csv.gz 101.2 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_gsea_H.csv.gz 5.1 Kb (ftp)(http) CSV
GSE233743_Exp3_Trt3.vs.Disease_top_hits.csv.gz 980.6 Kb (ftp)(http) CSV
GSE233743_Exp3_process_count_data_salmon.html.gz 3.9 Mb (ftp)(http) HTML
GSE233743_Exp3_sample_list.docx.gz 11.1 Kb (ftp)(http) DOCX
GSE233743_FC_comparison.xlsx 1.2 Mb (ftp)(http) XLSX
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