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| Status |
Public on Nov 10, 2023 |
| Title |
Cancer immunotherapy via synergistic co-activation of myeloid cells [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to define the impact of beta-glucan (CLEC7A agonist), anti-CD40 (agonist) and the combination of beta-glucan and anti-CD40 on the intra-tumoral immune landscape of mouse orthotopic pancreatic tumors using single-cell RNA sequencing.
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| Overall design |
This repository contains single-cell RNA sequencing of mouse (PDA.7940B mouse tumor cells) (n=2 samples/condition) untreated or 8 days post-treatment with beta-glucan, anti-CD40 or beta-glucan and anti-CD40. Two samples (consisting of 2-3 pooled tumors) per treatment condition were processed. Tissues were mechanically minced and digested with collagenase/DNase and filtered. Dead cells were removed using a Dead cell removal kit (Miltenyi) and single-cell cDNA libraries were prepared at the CHOP Single Cell Genomics Core at the University of Pennsylvania.
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| Contributor(s) |
Wattenberg MM, Coho H, Herrara VM, Graham K, Stone ML, Xue Y, Chang RB, Cassella C, Liu M, Choi-Bose S, Thomas SK, Choi H, Li Y, Melendez L, Markowitz K, Giannone M, Bose N, Beatty GL |
| Citation(s) |
37976347 |
| |
| Submission date |
May 22, 2023 |
| Last update date |
Feb 10, 2024 |
| Contact name |
Max M Wattenberg |
| E-mail(s) |
max.wattenberg@pennmedicine.upenn.edu
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| Organization name |
University of Pennsylvania
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| Lab |
Beatty
|
| Street address |
3400 Civic Center Blvd
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| City |
Philadelphia |
| State/province |
PENNSYLVANIA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE233175 |
Cancer immunotherapy via synergistic co-activation of myeloid cells |
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| Relations |
| BioProject |
PRJNA975171 |
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