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| Status |
Public on Jun 01, 2024 |
| Title |
A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survival [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Gene deserts spanning more than 500kb of non-protein coding genomic sequence are considered evolutionarily ancient and stable and are enriched in the vicinity of developmental regulator genes (Ovcharenko 2005). These extensive genomic regions typically harbor numerous conserved elements with predicted gene regulatory potential pointing to critical tissue-specific functions during development. Nevertheless, the biological necessity and underlying funtional enhancer landscapes of most gene deserts near developmental transcription factors (TFs) remain unknown, and it is unclear how precise pleiotropic expression patterns emerge from gene desert sequence. Here, we investigated the cis-regulatory architecture and function of a gene desert flanking the mouse Shox2 transcriptional regulator which itself is essential for embryonic limb, craniofacial, and cardiac pacemaker development. By combining epigenomic enhancer prediction, transgenic reporter validation and region-specific chromatin capture (C-HiC), we define the embryonic in vivo enhancer landscape and chromatin topology of the Shox2 gene desert. Targeted and context-specific genomic deletions uncover the gene desert not only as a regulator of embryonic survival through enhancer-mediated control of cardiac Shox2 expression, but also link distinct subsets of tissue-specific gene desert enhancers to the regulation of craniofacial patterning and proximal limb development. Our results hence identify the Shox2 gene desert as a fundamental genomic unit indispensable for pleiotropic patterning, robust organ morphogenesis and embryonic development progression by serving as a dynamic hub for tissue-specific developmental enhancers.
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| Overall design |
ATAC-seq from whole-mount mouse embryonic hearts to determine genome-wide cardiac open chromatin regions
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| Contributor(s) |
Zoia M, Mannion B, Barozzi I, Visel A, Pennacchio L, Osterwalder M |
| Citation missing |
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| |
| Submission date |
May 19, 2023 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Marco Osterwalder |
| E-mail(s) |
olheartdev@gmail.com
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| Organization name |
Universiy of Bern
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| Department |
DBMR
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| Lab |
Osterwalder Lab
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| Street address |
Murtenstrasse 24
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| City |
Bern |
| ZIP/Postal code |
3008 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE232887 |
A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survival |
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| Relations |
| BioProject |
PRJNA974367 |
