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Series GSE232861 Query DataSets for GSE232861
Status Public on Jul 20, 2023
Title Recapitulating human peri-gastrulation development via self-organization of extended pluripotent stem cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Stem cell models that replicate the gastrulation process in human embryos have been created, but they lack the essential extraembryonic cells needed for early embryonic development and patterning. Here, we introduce a robust and efficient method that prompts human extended pluripotent stem (EPS) cells to self-organize into embryo-like structures, called peri-gastruloids, which encompass both embryonic (epiblast) and extraembryonic (hypoblast) tissues. These peri-gastruloids simulate critical stages of human peri-gastrulation development, such as forming amniotic and yolk sac cavities, developing bilaminar and trilaminar embryonic discs, specifying primordial germ cells, initiating gastrulation, and early neurulation. Single-cell RNA sequencing unveiled transcriptomic characteristics of these human peri-gastruloids, which closely resemble the primary peri-gastrulation cell types found in human and non-human primates. Our results emphasize the remarkable self-organizing ability of EPS cells to generate advanced human embryo-like structures. This peri-gastruloid platform allows for further exploration beyond gastrulation and may potentially aid in the development of human fetal tissues for use in regenerative medicine.
 
Overall design Peri-gastruloids were pooled and harvested after 11 days of development for scRNA-seq analysis
Web link https://pubmed.ncbi.nlm.nih.gov/37478861/
 
Contributor(s) Liu L, Wang L
Citation(s) 37478861
Submission date May 18, 2023
Last update date Oct 27, 2023
Contact name Jun Wu
E-mail(s) jun2.wu@utsouthwestern.edu
Organization name University of Texas Southwestern Medical Center
Department Department of Molecular Biology
Lab Wu lab
Street address 5323 harry Hines Blvd.
City Dallas
State/province Texas
ZIP/Postal code 75235
Country USA
 
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (1)
GSM7384960 peri-gastruloid, day 11, rep1
Relations
BioProject PRJNA974153

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Supplementary file Size Download File type/resource
GSE232861_RAW.tar 53.4 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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