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| Status |
Public on Jan 25, 2024 |
| Title |
Spatially-resolved transcriptomics reveal macrophage heterogeneity and prognostic significance in diffuse large B-cell lymphoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages are abundant immune cells in the microenvironment of diffuse large B-cell lymphoma (DLBCL). Macrophage phenotyping by immunohistochemistry has varying prognostic significance across studies in DLBCL and does not provide a comprehensive analysis of macrophage subtypes. We hypothesized that whole-transcriptomic analysis (WTA) of macrophage in-situ would identify new macrophage subsets of biological and clinical significances.
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| Overall design |
Using digital spatial profiling with whole transcriptome analysis of CD68+ cells, we characterized macrophages in distinct spatial niches of reactive lymphoid tissues (RLTs) and DLBCL. We reveal previously unrecognized transcriptomic differences between macrophages populating in RLTs (light zone (LZ)/ dark zone (DZ), germinal center (GC)/ interfollicular (IF) regions), and in between disease states (RLTs and DLBCL). And we also captured the CD20+ cells in the same way, which was used for validation.
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| Contributor(s) |
Liu M, Jaynes P, Hue SS, Ng S, Tripodo C, Jeyasekharan AD |
| Citation(s) |
38459052 |
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| Submission date |
May 18, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Min Liu |
| E-mail(s) |
liuminnus@gmail.com
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| Organization name |
National University of Singapore
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| Street address |
13-02, 14 Medical Drive, Singapore
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| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
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| Platforms (2) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (592)
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| Relations |
| BioProject |
PRJNA974073 |
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