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| Status |
Public on Oct 13, 2023 |
| Title |
Mild dehydration effects on the murine kidney single nucleus transcriptome and chromatin accessibility |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The kidney is composed of > 40 cell types that work collectively to maintain fluid-electrolyte balance. If not enough fluid is consumed to remain in balance, then vasopressin is released and acts in the kidney to increase water reabsorption. Here the authors used kidney single nucleus multiome-sequencing to determine cell-specific transcriptomes and chromatin accessibility profiles of male and female control (ad libitum water and food) or mildly dehydrated mice (ad libitum food, water deprivation). There were hundreds of sex- and dehydration-specific differentially expressed genes throughout the kidney, most of which did not have a significant change in chromatin accessibility. Thus, even mild dehydration causes cell- and sex-specific transcriptomic changes even though the kidney function to conserve water is the same.
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| Overall design |
All are C57BL/6J mice. A male ad lib, a male water deprived, a female ad lib and a female mouse water deprived. Water was removed from the cages of the deprived mice at 3 pm. All sample collection occurred at +/-1 h at midnight (6 hours into the active period of the mice).
630 Female, Ad lib
636 Female, water deprived
638 Male, water deprived
641 Male, Ad lib
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| Contributor(s) |
Hyndman KA, Huynh NV |
| Citation(s) |
37767569 |
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| Submission date |
May 16, 2023 |
| Last update date |
Jan 12, 2024 |
| Contact name |
Kelly Anne Hyndman |
| E-mail(s) |
hyndmank@uab.edu
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| Organization name |
University of Alabama at Birmingham
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| Department |
Medicine
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| Lab |
Hyndman
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| Street address |
1918 University BLVD
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| City |
Birmingham |
| State/province |
Al |
| ZIP/Postal code |
35233 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA973115 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE232662_barcodes.xlsx |
388.7 Kb |
(ftp)(http) |
XLSX |
| GSE232662_combined.rds.gz |
7.1 Gb |
(ftp)(http) |
RDS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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