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Series GSE232335 Query DataSets for GSE232335
Status Public on Apr 04, 2024
Title Single cell gene expression profile of tumor-associated fibroblasts from macrophage-depleted PDAC liver metastasis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used single cell RNA sequencing to investigate how macrophages regulate tumor-associated fibroblast heterogeneity in PDAC liver metastasis
Overall design Experimental liver metastasis in Pdgfrb-GFP reporter mouse model for 14 days. Macrophages were depleted with CSF1R neutralizing antibody, or IgG control, from Day 7 to 14. GFP+ metastasis-associated fibroblasts and GFP+ cells from naive liver were enriched by fluorescence activated cell sorting (FACS) and analyzed by single cell RNA sequencing
Contributor: Centre for Genomics Research, Liverpool
Contributor(s) Raymant M, Astuti Y, Green D, Schmid M
Citation(s) 38678021
Submission date May 11, 2023
Last update date May 24, 2024
Contact name Michael C Schmid
Organization name University of Liverpool
Department Molecular and Clinical Cancer Medicine
Lab Schmid Lab
Street address Ashton Street
City Liverpool
State/province Merseyside
ZIP/Postal code L69 3GE
Country United Kingdom
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (3)
GSM7328793 Liver, Naïve
GSM7328794 Liver, IgG
GSM7328795 Liver, anti-CSF1R
BioProject PRJNA971635

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Supplementary file Size Download File type/resource
GSE232335_RAW.tar 324.9 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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