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Status |
Public on Apr 04, 2024 |
Title |
Single cell gene expression profile of tumor-associated fibroblasts from macrophage-depleted PDAC liver metastasis |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We used single cell RNA sequencing to investigate how macrophages regulate tumor-associated fibroblast heterogeneity in PDAC liver metastasis
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Overall design |
Experimental liver metastasis in Pdgfrb-GFP reporter mouse model for 14 days. Macrophages were depleted with CSF1R neutralizing antibody, or IgG control, from Day 7 to 14. GFP+ metastasis-associated fibroblasts and GFP+ cells from naive liver were enriched by fluorescence activated cell sorting (FACS) and analyzed by single cell RNA sequencing Contributor: Centre for Genomics Research, Liverpool
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Contributor(s) |
Raymant M, Astuti Y, Green D, Schmid M |
Citation(s) |
38678021 |
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Submission date |
May 11, 2023 |
Last update date |
May 24, 2024 |
Contact name |
Michael C Schmid |
E-mail(s) |
mschmid@liverpool.ac.uk
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Organization name |
University of Liverpool
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Department |
Molecular and Clinical Cancer Medicine
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Lab |
Schmid Lab
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Street address |
Ashton Street
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City |
Liverpool |
State/province |
Merseyside |
ZIP/Postal code |
L69 3GE |
Country |
United Kingdom |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (3) |
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Relations |
BioProject |
PRJNA971635 |