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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 13, 2023 |
| Title |
Circadian ribosome profiling reveals a role for the Period2 upstream opening reading frame in sleep. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Many mammalian proteins have circadian cycles of production and degradation, and many of these rhythms are altered post-transcriptionally. We used ribosome profiling to examine post-transcriptional control of circadian rhythms by quantifying RNA translation in the liver over a 24-h period from circadian-entrained mice transferred to constant darkness conditions and by comparing ribosome binding levels to protein levels for 16 circadian proteins. We observed large differences in ribosome binding levels compared to protein levels, and we observed delays between peak ribosome binding and peak protein abundance. We found extensive binding of ribosomes to upstream open reading frames (uORFs) in circadian mRNAs, including the core clock gene Period2 (Per2). An increase in the number of uORFs in the 5'UTR was associated with a decrease in ribosome binding in the main coding sequence and a reduction in expression of synthetic reporter constructs. Mutation of the Per2 uORF increased luciferase and fluorescence reporter expression in 3T3 cells without altering the phase or period and increased luciferase expression in PER2:LUC MEF cells. Mutation of the Per2 uORF in mice increased PER2 protein levels and reduced total sleep time compared to that in wild-type mice. These results suggest that post-transcriptional processes shape translation of mRNA transcripts, which can impact physiological rhythms and sleep.
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| Overall design |
Ribosome profiling and RNA sequencing of three C57BL/6N mouse livers from wild-type and Per2uORF mutant mice
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| Web link |
https://www.pnas.org/doi/10.1073/pnas.2214636120
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| Contributor(s) |
Millius A, Perrin D, Ueda HR |
| Citation(s) |
37769257 |
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| Submission date |
May 05, 2023 |
| Last update date |
Dec 13, 2023 |
| Contact name |
Arthur Millius |
| E-mail(s) |
arthur.millius@ifrec.osaka-u.ac.jp
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| Phone |
+81-06-6879-8366
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| Organization name |
Osaka University
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| Department |
iFReC
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| Lab |
Systems Immunology
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| Street address |
iFReC 6F
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| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| GSM7304543 |
Per2uORF mutant male, RNAseq ZT02-04, rep1 |
| GSM7304544 |
Per2uORF mutant male, RNAseq ZT02-04, rep2 |
| GSM7304545 |
Per2uORF mutant male, RNAseq ZT02-04, rep3 |
| GSM7304546 |
Wild-type male, RiboProf ZT02-04, rep1 |
| GSM7304547 |
Wild-type male, RiboProf ZT02-04, rep2 |
| GSM7304548 |
Wild-type male, RiboProf ZT02-04, rep3 |
| GSM7304549 |
Per2uORF mutant male, RiboProf ZT02-04, rep1 |
| GSM7304550 |
Per2uORF mutant male, RiboProf ZT02-04, rep2 |
| GSM7304551 |
Per2uORF mutant male, RiboProf ZT02-04, rep3 |
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| Relations |
| BioProject |
PRJNA967742 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231820_DatasetS4.xlsx |
5.7 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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