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Series GSE231507 Query DataSets for GSE231507
Status Public on Apr 15, 2024
Title Impact of azide metabolic glycoengineering and bioorthogonal click chemistry cell-cell tethering in adipose tissue-derived mesenchymal stromal cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report RNA sequencing data from adipose tissue-derived mesenchymal stromal cells (hASCs) functionalized via metabolic glycoengineering with pendant azide sialosides. Beyond the impact of this cell surface engineering on transcriptome, we explore the expression profiles of azide-hASCs covalently tethered to an interfacial glycosaminoglycan via bioorthogonal click chemistry and yielding a 3D multicellular construct. We find global gene expression changes in response to metabolic glycoengineering in 2D cell culture conditions as well as those arising from cell-to-cell tethering into 3D environments.
Overall design Comparative gene expression profiling analysis of RNA-seq data for hASCs in three conditions (2D Native, 2D Glycoengineered and 3D Day 1 Cellgels)
Contributor(s) Lavrador P, Moura BS, Almeida-Pinto J, Gaspar VM, Mano JF
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BioProject PRJNA949992
Submission date May 02, 2023
Last update date Apr 15, 2024
Contact name Pedro Lavrador
Organization name University of Aveiro
Department CICECO - Aveiro Institute of Materials
Street address Campus Universitário de Santiago, Universidade de Aveiro
City Aveiro
ZIP/Postal code 3810-193
Country Portugal
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (15)
GSM7286937 2D_Native_1
GSM7286938 2D_Native_2
GSM7286939 2D_Native_3

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE231507_2D_normalizedcounts.csv.gz 1.0 Mb (ftp)(http) CSV
GSE231507_2Dvs3D_normalizedcounts.csv.gz 1.0 Mb (ftp)(http) CSV
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