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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 13, 2023 |
| Title |
Dietary protein shapes the profile and repertoire of intestinal CD4+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
This study shows in a polyclonal setting that routine exposure to food protein promotes accumulation of antigen-experienced CD4+ T cells in the intestinal epithelium, which acquire a cytotoxic tissue-resident transcriptional program. This process is disrupted upon either allergic sensitization or tolerance.
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| Overall design |
Single CD4+ T cells from the small intestine epithelium or lamina propria of mice with different dietary and microbial manipulations; libraries are the antibody derived tags, gene expression and tcr sequencing.
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| Contributor(s) |
Lockhart A, Reed A, Castro TB, Herman C, Canesso MC, Mucida D |
| Citation(s) |
37191720 |
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| Submission date |
Apr 30, 2023 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Tiago Castro |
| E-mail(s) |
trezende@rockefeller.edu
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| Organization name |
The Rockefeller University
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| Lab |
Laboratory of Mucosal Immunology
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| Street address |
1230 York Avenue
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (15)
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| GSM7265577 |
Small intestine CD4 from GF mice fed AA or OVA diet - Hashtags |
| GSM7265578 |
Small intestine CD4 from GF mice fed AA or OVA diet - Gex |
| GSM7265579 |
Small intestine CD4 from GF mice fed AA or OVA diet - TCR |
| GSM7265580 |
Small intestine CD4 from MM12 mice fed AA, OVA, or chow diet - Hashtags |
| GSM7265581 |
Small intestine CD4 from MM12 mice fed AA, OVA, or chow diet - Gex |
| GSM7265582 |
Small intestine CD4 from MM12 mice fed AA, OVA, or chow diet - TCR |
| GSM7265583 |
Small intestine CD4 from GF mice fed OTIIp or Chow diet - Hashtags |
| GSM7265584 |
Small intestine CD4 from GF mice fed OTIIp or Chow diet - Gex |
| GSM7265585 |
Small intestine CD4 from GF mice fed OTIIp or Chow diet - TCR |
| GSM7265586 |
Small intestine CD4 from SellTomato mice in OVA feeding, tolerance, and allergy - Hashtags 1 |
| GSM7265587 |
Small intestine CD4 from SellTomato mice in OVA feeding, tolerance, and allergy - Gex 1 |
| GSM7265588 |
Small intestine CD4 from SellTomato mice in OVA feeding, tolerance, and allergy - TCR 1 |
| GSM7265589 |
Small intestine CD4 from SellTomato mice in OVA feeding, tolerance, and allergy - Hashtags 2 |
| GSM7265590 |
Small intestine CD4 from SellTomato mice in OVA feeding, tolerance, and allergy - Gex 2 |
| GSM7265591 |
Small intestine CD4 from SellTomato mice in OVA feeding, tolerance, and allergy - TCR 2 |
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| Relations |
| BioProject |
PRJNA964460 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231351_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of CSV, MTX, RDS, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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