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Series GSE231301 Query DataSets for GSE231301
Status Public on Feb 21, 2024
Title RNA sequencing of murine in vitro differentiated and repetitively activated, antigen-experienced TCF1+CD8+ T cells after PGE2 treatment and stimulation [bulk RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing was used to characterise PGE2-mediated changes in the gene expression profile of in vitro differentiated and repetitively activated antigen-experienced TCF1+CD8+ T cells.
 
Overall design In vitro differentiated and repetitively activated antigen-experienced TCF1+CD8+ T cells were pre-incubated with or without PGE2 (100 ng/mL) for 1 hour at 37°C. Subsequently, the cells were stimulated for an additional 4 hours with low dose IL-2 (85 U/ml) or the combination of low dose IL-2 and aCD3/CD28 beads.
 
Contributor(s) Böttcher J, Lacher S, de Almeida GP
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Submission date Apr 29, 2023
Last update date Feb 21, 2024
Contact name Gustavo P. de Almeida
E-mail(s) gustavo.almeida@tum.de
Organization name Technical University of Munich
Department Division of Animal Physiology and Immunology
Street address Weihenstephaner Berg 3
City Freising
State/province Bavaria
ZIP/Postal code 85354
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM7256665 TCF1+CD8+ T cells, no beads, IL-2, no PGE2, rep1
GSM7256666 TCF1+CD8+ T cells, no beads, IL-2, no PGE2, rep2
GSM7256667 TCF1+CD8+ T cells, no beads, IL-2, no PGE2, rep3
This SubSeries is part of SuperSeries:
GSE231340 PGE2 curtails IL-2-dependent effector expansion from tumour-infiltrating stem-like CD8+ T cells to promote cancer immune escape
Relations
BioProject PRJNA963167

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE231301_feature_counts.txt.gz 2.4 Mb (ftp)(http) TXT
GSE231301_gene_fpkm.txt.gz 3.8 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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