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Series GSE230805 Query DataSets for GSE230805
Status Public on Feb 21, 2024
Title Aging-associated Decline in Vascular Smooth Muscle Cell Mechanosensation is Mediated by Piezo1 Channel
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Aging of the vasculature is associated with detrimental changes in vascular smooth muscle cell (VSMC) mechanosensitivity to extrinsic forces in their surrounding microenvironment. However, how chronological aging alters VSMCs’ ability to sense and adapt to mechanical perturbations remains unexplored. Here, we show defective VSMC mechanosensation in aging measured with ultrasound tweezers-based micromechanical system, force instantaneous frequency spectrum and transcriptome analyses. The mechanobiological study reveals thataged VSMCs adapt a relatively inert solid-like state with altered actin cytoskeletal integrity, resulting in an impairment in their mechanosensitivity and dynamic mechanoresponse to mechanical perturbations. The aging-associated decline in mechanosensation behaviors is mediated by hyperactivity of Piezo1-dependent calcium signaling. Inhibition of Piezo1 alleviates vascular aging and partially restores the loss in dynamic contractile properties in aged cells. Altogether, our study reveals the novel signaling pathway underlying aging-associated aberrant mechanosensation in VSMC and identifies Piezo1 as a potential therapeutic mechanobiological target to alleviate vascular aging.
Overall design Single-cell RNA-sequencing libraries of four groups of vascular smooth muscle cells extracted from C57BL/6 mice young (12 weeks) and old (58-78 weeks) cultured in four different treatments. Young + Angiotensin II and Old + siPiezo1 are the experimental groups, while Young and Old are the control groups.
Contributor(s) Luu N, Chen W
Citation(s) 37941511
Submission date Apr 27, 2023
Last update date Feb 21, 2024
Contact name Ngoc Luu
Phone 2036009032
Organization name New York University
Street address 6 Metrotech Center
City Brooklyn
State/province NY
ZIP/Postal code 11201
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM7235283 VSMCs - GEX
GSM7235284 VSMCs - Hashtag
BioProject PRJNA962574

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Supplementary file Size Download File type/resource
GSE230805_barcodes.tsv.gz 7.7 Mb (ftp)(http) TSV
GSE230805_features.tsv.gz 254.2 Kb (ftp)(http) TSV
GSE230805_matrix.mtx.gz 226.9 Mb (ftp)(http) MTX
GSE230805_sample_manifest.xlsx 11.6 Kb (ftp)(http) XLSX
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