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Series GSE230792 Query DataSets for GSE230792
Status Public on Jan 22, 2024
Title Cis inhibition of NOTCH1 through JAGGED1 sustains embryonic Hematopoietic stem cell fate [scRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hematopoietic stem cells (HSCs) develop from the hemogenic endothelium (HE) in the aorta- gonads-and mesonephros (AGM) region and reside within Intra-aortic hematopoietic clusters (IAHC) along with hematopoietic progenitors (HPC). The signalling mechanisms that distinguish HSCs from HPCs are unknown. Notch signaling is essential for arterial specification, IAHC formation and HSC activity, but current studies on how Notch segregates these different fates are inconsistent.
We now demonstrate that Notch activity is highest in a subset of, GFI1+, HSC-primed HE cells, and is gradually lost with HSC maturation. We uncover that the HSC phenotype is maintained due to increasing levels of NOTCH1 and JAG1 interactions on the surface of the same cell (cis) that renders the NOTCH1 receptor from being activated. Forced activation of the NOTCH1 receptor in IAHC activates a hematopoietic differentiation program. Our results indicate that NOTCH1-JAG1 cis-inhibition preserves the HSC phenotype in the hematopoietic clusters of the embryonic aorta.

 
Overall design We performed single cell RNA seq with sorted AGM E11.5 and E10.5 CD31+ cKit+ GFI1+ with a total of 860 cells in order to define HSCs at a molecular level.
 
Contributor(s) Thambyrajah R, Maqueda M, Hao Neo W, Imbach K, Guillen Y, Grases D, Fadlullah Z, Gambera S, Matteini F, Wang X, Calero F, Esteller M, Florian MC, Porta E, Benedito R, Göttgens B, Lacaud G, Espinosa L, Bigas A
Citation(s) 38383534
Submission date Apr 27, 2023
Last update date Apr 22, 2024
Contact name Anna Bigas
E-mail(s) abigas@imim.es
Phone +34933160440
Organization name Institut Hospital del Mar d'Investigacions Mèdiques
Department Cancer Research
Lab Stem Cells and Cancer
Street address Dr. Aiguader 88
City Barcelona
State/province Barcelona
ZIP/Postal code 08003
Country Spain
 
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (860)
GSM7234364 GL53_001_Plate3_a1_Gfi1_HE_S1
GSM7234365 GL53_002_Plate3_b1_Gfi1_HE_S2
GSM7234366 GL53_003_Plate3_c1_Gfi1_HE_S3
This SubSeries is part of SuperSeries:
GSE230794 Cis inhibition of NOTCH1 through JAGGED1 sustains embryonic Hematopoietic stem cell fate
Relations
BioProject PRJNA962580

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE230792_scRNAseq_Cell_annotation_final.txt.gz 70.4 Kb (ftp)(http) TXT
GSE230792_scRNAseq_Norm_counts_filtered.txt.gz 25.9 Mb (ftp)(http) TXT
GSE230792_scRNAseq_Raw_counts_final.txt.gz 9.9 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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