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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 28, 2023 |
| Title |
Bidirectional changes in postmitotic H3K27me3 distributions underlie cerebellar granule neuron maturation dynamics [RNA-seq DIV3] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The functional and transcriptional maturation of neurons is a prolonged process that extends well beyond mitotic exit and terminal fate commitment of progenitors. The differentiation of cerebellar granule neurons (CGNs) in the postnatal mouse cerebellum provides a useful model to identify chromatin mechanisms that orchestrate temporal changes in transcription as postmitotic CGNs mature. Here we report that CGN maturation is associated with dynamic changes in the genomic distribution of histone H3 lysine 27 trimethylation (H3K27me3), a modification best known for its role in repressing alternative fates during cell specification. H3K27me3 is gained rapidly in newly postmitotic CGNs at progenitor-expressed genes that are repressed in neurons. H3K27me3 is lost more gradually at the promoters of a subset of neuronal genes that are transcriptionally induced upon CGN maturation. The loss of H3K27me3 is facilitated by the lysine demethylase KDM6B, and genes that are induced in maturing CGNs show impaired expression in the cerebellum of conditional KDM6B knockout mice. Genes that lose H3K27me3 gain H3K27 acetylation and binding of the pro-maturation ZIC1/2 transcription factors, suggesting that developmental loss of H3K27me3 may be required to permit the onset of transcriptional maturation. Interestingly, pharmacological inhibition of the H3K27 methyltransferase EZH2 in early postmitotic CGNs not only blocked the repression of progenitor genes but also impaired the induction of mature CGN genes. These data show that regulation of H3K27me3 functions in developing postmitotic neurons beyond the period of cell fate commitment to regulate the dynamics of gene expression programs that underlie functional neuronal maturation.
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| Overall design |
Cerebellum treated with DMSO or GSK-126, at DIV3.
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| Contributor(s) |
Ramesh V, Liu F, Minto M, Chan U, West AE |
| Citation(s) |
37092728 |
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| Submission date |
Apr 27, 2023 |
| Last update date |
Apr 30, 2023 |
| Contact name |
Anne E West |
| E-mail(s) |
west@neuro.duke.edu
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| Organization name |
Duke University
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| Department |
Neurobiology
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| Lab |
West Lab
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| Street address |
311 Research Dr
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27705 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE212441 |
Bidirectional changes in postmitotic H3K27me3 distributions underlie cerebellar granule neuron maturation dynamics |
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| Relations |
| BioProject |
PRJNA962332 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230712_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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