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| Status |
Public on Jul 22, 2024 |
| Title |
Innate immune memory after brain injury drives inflammatory cardiac dysfunction [snATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Besides the local inflammatory immune response in the brain, stroke also alters systemic immunity. Within the acute and sub-acute phase, the systemic immune response to stroke has been described in detail over last decades. The long-term systemic immunological consequences after stroke are however still elusive. Therefore, a better understanding of these long-lasting chronic effects of stroke on systemic immunity and its impact on remote organ function and secondary comorbidities is needed. Here, we used single-cell RNA sequencing (scRNA-Seq) to investigate the chronic effect of stroke on the transcriptomic signatures of resident myeloid immune cells in various peripheral organs remote from the brain, including the lung, heart, liver, spleen, blood and bone marrow. We observed that monocytes in peripheral organs and in the bone marrow adopt a pro-inflammatory phenotype which persists chronically after stroke. Moreover, the pro-inflammatory profile of monocytes in the bone marrow was transmissible by BM transplantation to naïve recipients, indicating potentially epigenetically imprinted chronic innate immune memory after stroke. Indeed, by performing single-nuclei ATAC sequencing, we found that post-stroke myeloid immune memory after stroke is likely mediated by IL-1b-driven mechanisms, and that neutralization of the post-stroke increase in circulating IL-1b prevented myeloid immune memory.
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| Overall design |
Mouse single-nuclei ATAC experiment performed on myeloid cells from bone marrow chronically after stroke and control conditions. An additional group of bone marrow cells from stroke mice treated with neutralizing antibodies against IL-1b was also included (stroke-treated group).
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| Contributor(s) |
Simats A, Zhang S, Messerer D, Chong F, Beskardes S, Chivukula A, Cao J, Besson-Girard S, Montellano F, Morbach C, Carofiglio O, Ricci A, Roth S, Llovera G, Singh R, Chen Y, Filser S, Plesnila N, Braun C, Spitzer H, Gocke O, Dichgans M, Heuschmann P, Hatakeyama K, Beltrán E, Clauss S, Bonev B, Schulz C, Liesz A |
| Citation missing |
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| Submission date |
Apr 26, 2023 |
| Last update date |
Jul 22, 2024 |
| Contact name |
Arthur Liesz |
| E-mail(s) |
lieszlabmunich@gmail.com
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| Organization name |
LMU Hospital
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| Department |
Institute for Stroke and Dementia (ISD)
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| Street address |
Feodor-Lynen-Straße 17
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| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (8)
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| GSM7232712 |
Bone marrow, Stroke-treated, snATACseq, rep1 |
| GSM7232713 |
Bone marrow, Control, snATACseq, rep2 |
| GSM7232714 |
Bone marrow, Stroke, snATACseq, rep3 |
| GSM7232715 |
Bone marrow, Stroke-treated, snATACseq, rep2 |
| GSM7232716 |
Bone marrow, Stroke-treated, snATACseq, rep3 |
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| Relations |
| BioProject |
PRJNA962132 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230692_barcodes.tsv.gz |
223.1 Kb |
(ftp)(http) |
TSV |
| GSE230692_fragments.tsv.gz.tbi.gz |
1.7 Mb |
(ftp)(http) |
TBI |
| GSE230692_matrix.mtx.gz |
656.6 Mb |
(ftp)(http) |
MTX |
| GSE230692_peaks.bed.gz |
2.1 Mb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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