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GEO help: Mouse over screen elements for information. |
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Status |
Public on May 31, 2023 |
Title |
microRNA-132 regulates gene expression programs involved in microglial homeostasis |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
microRNA-132 (miR-132), a known neuronal regulator, is one of the most robustly downregulated microRNAs (miRNAs) in the brain of Alzheimer’s disease (AD) patients. Increasing miR-132 in AD mouse brain ameliorates amyloid and Tau pathologies, and also restores adult hippocampal neurogenesis and memory deficits. However, the functional pleiotropy of miRNAs requires in-depth analysis of the effects of miR-132 supplementation before it can be moved forward for AD therapy. We employ here miR-132 loss- and gain-of-function approaches using single-cell transcriptomics, proteomics and in silico AGO-CLIP datasets to identify molecular pathways targeted by miR-132 in mouse hippocampus. We find that miR-132 modulation significantly affects the transition of microglia from a disease-associated to a homeostatic cell state. We confirm the regulatory role of miR-132 in shifting microglial cell states using human microglial cultures derived from induced pluripotent stem cells.
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Overall design |
Cells from the right hippocampus of mice treated with miR-132 antagomiR/scrambled control and miR-132 mimic/negative control were isolated by Fluorescence-activated cell sorting (FACS) based on viability (e780, replicates 1 and 3) and in addition for the presence or absence of YFP signal (neurons) or CD11b and ACSA-2 (astrocytes) (replicates 2, 4 and 5) and analyzed using scRNAseq.
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Contributor(s) |
Walgrave H, Penning A, Tosoni G, Snoeck S, Davie K, Davis E, Wolfs L, Sierksma A, Mars M, Bu T, Thrupp N, Zhou L, Moechars D, Mancuso R, Fiers M, Howden AJ, De Strooper B, Salta E |
Citation(s) |
37250784 |
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Submission date |
Apr 21, 2023 |
Last update date |
Jun 01, 2023 |
Contact name |
Kristofer James Davie |
Organization name |
Vlaams Instituut voor Biotechnologie
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Department |
Center for Brain and Disease Research
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Street address |
Herestraat 49
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City |
Leuven |
ZIP/Postal code |
3000 |
Country |
Belgium |
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Platforms (2) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (16)
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GSM7213986 |
Control OE, Run1, scRNA-seq |
GSM7213987 |
miR-132 KD, Run2, scRNA-seq |
GSM7213988 |
Control KD, Run2, scRNA-seq |
GSM7213989 |
miR-132 OE, Run2, scRNA-seq |
GSM7213990 |
Control OE, Run2, scRNA-seq |
GSM7213991 |
miR-132 KD, Run3, scRNA-seq |
GSM7213992 |
Control KD, Run3, scRNA-seq |
GSM7213993 |
miR-132 OE, Run4, scRNA-seq |
GSM7213994 |
Control OE, Run4, scRNA-seq |
GSM7213995 |
miR-132 KD, Run5, scRNA-seq |
GSM7213996 |
Control KD, Run5, scRNA-seq |
GSM7213997 |
miR-132 OE, Run5, scRNA-seq |
GSM7213998 |
Control OE, Run5, scRNA-seq |
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Relations |
BioProject |
PRJNA958254 |
Supplementary file |
Size |
Download |
File type/resource |
GSE230333_RAW.tar |
239.8 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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