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Series GSE230333 Query DataSets for GSE230333
Status Public on May 31, 2023
Title microRNA-132 regulates gene expression programs involved in microglial homeostasis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary microRNA-132 (miR-132), a known neuronal regulator, is one of the most robustly downregulated microRNAs (miRNAs) in the brain of Alzheimer’s disease (AD) patients. Increasing miR-132 in AD mouse brain ameliorates amyloid and Tau pathologies, and also restores adult hippocampal neurogenesis and memory deficits. However, the functional pleiotropy of miRNAs requires in-depth analysis of the effects of miR-132 supplementation before it can be moved forward for AD therapy. We employ here miR-132 loss- and gain-of-function approaches using single-cell transcriptomics, proteomics and in silico AGO-CLIP datasets to identify molecular pathways targeted by miR-132 in mouse hippocampus. We find that miR-132 modulation significantly affects the transition of microglia from a disease-associated to a homeostatic cell state. We confirm the regulatory role of miR-132 in shifting microglial cell states using human microglial cultures derived from induced pluripotent stem cells.
 
Overall design Cells from the right hippocampus of mice treated with miR-132 antagomiR/scrambled control and miR-132 mimic/negative control were isolated by Fluorescence-activated cell sorting (FACS) based on viability (e780, replicates 1 and 3) and in addition for the presence or absence of YFP signal (neurons) or CD11b and ACSA-2 (astrocytes) (replicates 2, 4 and 5) and analyzed using scRNAseq.
 
Contributor(s) Walgrave H, Penning A, Tosoni G, Snoeck S, Davie K, Davis E, Wolfs L, Sierksma A, Mars M, Bu T, Thrupp N, Zhou L, Moechars D, Mancuso R, Fiers M, Howden AJ, De Strooper B, Salta E
Citation(s) 37250784
Submission date Apr 21, 2023
Last update date Jun 01, 2023
Contact name Kristofer James Davie
Organization name Vlaams Instituut voor Biotechnologie
Department Center for Brain and Disease Research
Street address Herestraat 49
City Leuven
ZIP/Postal code 3000
Country Belgium
 
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM7213983 miR-132 KD, Run1, scRNA-seq
GSM7213984 Control KD, Run1, scRNA-seq
GSM7213985 miR-132 OE, Run1, scRNA-seq
Relations
BioProject PRJNA958254

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Supplementary file Size Download File type/resource
GSE230333_RAW.tar 239.8 Mb (http)(custom) TAR (of CSV, MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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