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Status |
Public on Sep 13, 2023 |
Title |
Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia gene intraflagellar transport homolog 88 in Alzheimer's disease |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
This study identified the specific types of hippocampal neurons whose axons are vulnerable to amyloid-β accumulation. We used the inDropsTM platform to sequence 8 libraries: 5 libraries from the hippocampus of Alzheimer’s disease mice at 12,20, and, 24 weeks of age, and 3 libraries from the hippocampus of normal age-matched mice for the purpose of finding the selectively vulnerable hippocampal neuron type. 22,233 cells with 72,146 transcriptomes were collected after quality control was completed. When the 5,468 neurons that were marked by the expression of Snap25, Syt1, Ndrg4, and Meg3 were examined, we found that three types of neurons, such as somatostatin, chromogranin B, and calbindin-expressing neurons in the hippocampus, were selectively vulnerable to amyloid-β overexpression.
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Overall design |
We used the inDropsTM platform to sequence 8 libraries: 5 libraries from the hippocampus of Alzheimer’s disease mice at 12,20, and, 24 weeks of age, and 3 libraries from the hippocampus of normal age-matched mice for the purpose of finding the selectively vulnerable hippocampal neuron type.
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Contributor(s) |
Jang J, Yeo S, Jung H, Lee M, Ba S, Choe Y |
Citation(s) |
37667395, 37942288 |
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Submission date |
Apr 21, 2023 |
Last update date |
Dec 01, 2023 |
Contact name |
Jaemyung Jang |
Organization name |
Korea Brain Research Institute
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Street address |
61, Cheomdan-ro, Dong-gu
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City |
Daegu |
ZIP/Postal code |
41062 |
Country |
South Korea |
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Platforms (2) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (8)
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GSM7208892 |
5xFAD, expr 1, scRNAseq |
GSM7208893 |
5xFAD, expr 2, scRNAseq |
GSM7208894 |
5xFAD, expr 3, scRNAseq |
GSM7208896 |
5xFAD, expr 4, scRNAseq |
GSM7208897 |
5xFAD, expr 5, scRNAseq |
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Relations |
BioProject |
PRJNA958109 |
Supplementary file |
Size |
Download |
File type/resource |
GSE230240_RAW.tar |
60.6 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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