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Series GSE230009 Query DataSets for GSE230009
Status Public on Nov 22, 2023
Title TGF-β induces matrisome pathological alterations and EMT in patient-derived prostate cancer tumoroids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Extracellular matrix (ECM) tumorigenic alterations resulting in high matrix deposition and stiffening are hallmarks of adenocarcinomas and are collectively defined as desmoplasia.
Here, we thoroughly analysed primary prostate cancer tissues obtained from numerous patients undergoing radical prostatectomy to highlight reproducible structural changes in the ECM leading to the loss of the glandular architecture.
Starting from patient cells, we established prostate cancer tumoroids (PCTs) and demonstrated they require TGF-β signalling pathway activity to preserve phenotypical and structural similarities with the tissue of origin.
By modulating TGF-β signalling pathway in PCTs, we unveiled its role in ECM accumulation and remodelling in prostate cancer.
We also found that TGF-β-induced ECM remodelling is responsible for the initiation of prostate cell epithelial-to-mesenchymal transition (EMT) and the acquisition of a migratory, invasive phenotype.
Our findings highlight the cooperative role of TGF-β signalling and ECM desmoplasia in prompting prostate cell EMT and promoting tumour progression and dissemination.
Overall design Comparative gene expression analysis of RNA-seq data obtained from prostate cancer organoids treated in the presence or absence of TGF-B signalling pathway inhibitor (A-83-01). Organoids isolated from 5 different donors were used for this study.
Web link
Contributor(s) Fernandes S, Oliver-De La Cruz J, Cassani M, Morazzo S, Ďuríková H, Caravella A, Fiore P, Azzato G, De Marco G, Lauria A, Izzi V, Bosáková V, Fric J, Filipensky P, Forte G, Hejret V
Citation(s) 37944712
Submission date Apr 18, 2023
Last update date Nov 23, 2023
Contact name Philipp Arnold
Organization name Institute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen-Nürnberg
Street address Universitätsstraße 19
City Erlangen
ZIP/Postal code 91054
Country Germany
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (10)
GSM7182698 Patient 32 organoids treated with TGF-B signalling pathway inhibitor A 83-01
GSM7182699 Patient 33 organoids treated with TGF-B signalling pathway inhibitor A 83-01
GSM7182700 Patient 35 organoids treated with TGF-B signalling pathway inhibitor A 83-01
BioProject PRJNA956959

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Supplementary file Size Download File type/resource
GSE230009_RAW.tar 43.7 Mb (http)(custom) TAR (of TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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