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Series GSE229892 Query DataSets for GSE229892
Status Public on Apr 21, 2023
Title Paired single nucleus RNA and ATAC sequencing on the role of diet and aging in hematopoietic stem and progenitor cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We performed joint single-nucleus RNA (snRNA-seq) and ATAC (snATAC-seq) sequencing on hematopoietic stem and progenitor cells (LSK) isolated from mouse bone marrow of mice of different ages and dietary treatments. Mice were either fed ad libitum continually until bone marrow isolation at young (yAL) or old (oAL) age, or underwent a mild dietary restriction (DR; 30% reduction in food intake by weight) for the two weeks prior to bone marrow isolation at young (yDR) or old (oDR) age to investigate early response to nutrient deprivation. Each of these four samples contained pooled cells from two mice.
 
Overall design Profiles of single-nucleus transcriptomes and epigenomes, assayed using snRNA-seq and scATAC-seq, across sorted LSK cells from mouse bone marrow for mice of different diets and ages.
 
Contributor(s) Behrends M, Chen Y, Bens M
Citation(s) 37131596
Submission date Apr 17, 2023
Last update date Aug 02, 2023
Contact name Marthe Behrends
Organization name Leibniz Institute on Aging – Fritz Lipmann Institute
Lab Rudolph
Street address Beutenbergstraße 11
City Jena
ZIP/Postal code 07745
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM7179771 hematopoietic stem and progenitor cells, old mice fed ad libitum , scRNAseq
GSM7179772 hematopoietic stem and progenitor cells, old diet restricted mice , scRNAseq
GSM7179773 hematopoietic stem and progenitor cells, young mice fed ad libitum , scRNAseq
Relations
BioProject PRJNA956541

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Supplementary file Size Download File type/resource
GSE229892_RAW.tar 7.4 Gb (http)(custom) TAR (of TAR, TBI, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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