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| Status |
Public on Sep 21, 2010 |
| Title |
Genome-wide expression analysis in kyp and cmt3 mutants of Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of genome-wide expression in the mutant of histone H3K9 methyltransferase KRYPTONITE (KYP) or DNA methyltransferase CHROMOMETHYLASE3 (CMT3) in Arabidopsis. These mutants showed decrease in H3K9 methylation and DNA methylation levels, and transcriptional activation at transposons and repeats. Using NimbleGen DNA microarray, global pattern of expression of genes and transposons were examined in these mutants.
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| Overall design |
Total RNA from leaves was isolated using the RNeasy Plant Mini kit (Qiagen) and was treated with DNase I (TAKARA). Double-stranded cDNA was synthesized using the SuperScript Double Stranded cDNA Synthesis kit (Invitrogen) and oligo (dT)20 primer. cDNA was labeled by Cy3, hybridized to 4 x 72k array, and scanned according to manufacture's instructions (www.nimblegen.com).
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| Contributor(s) |
Inagaki S, Kakutani T |
| Citation(s) |
20834229 |
| |
| Submission date |
Jul 15, 2010 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Soichi Inagaki |
| E-mail(s) |
soinagak@bs.naist.jp
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| Organization name |
Nara Institute of Science and Technology
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| Department |
Graduate School of Biological Sciences
|
| Street address |
8916-5, Takayama-cho
|
| City |
Ikoma |
| State/province |
Nara |
| ZIP/Postal code |
630-0192 |
| Country |
Japan |
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| Platforms (1) |
| GPL10689 |
NimbleGen Arabidopsis thaliana 4x72K Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE23030 |
Autocatalytic differentiation of epigenetic modifications within the Arabidopsis genome |
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| Relations |
| BioProject |
PRJNA133095 |
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