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| Status |
Public on Jul 11, 2023 |
| Title |
Synchronized response of CD4+ T cells to short-term dietary changes - Tissue RNA sequencing diet change |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Omnivorous animals, including mice and humans, tend to prefer energy-dense nutrients rich in fat over plant-based diets, especially for short periods of time. The health consequences of this short-term consumption of energy-dense nutrients remain still unclear. We found that every short-term, reiterated switches to feast diets mimicking our social eating behavior, breached the potential buffering effect of the intestinal microbiota and deeply reorganized the immunological architecture of mucosa-associated lymphoid tissues. The first dietary switch was sufficient to induce transient mucosal immune depression and suppress systemic, antigen-specific immunity leading to higher susceptibility to Salmonella Typhimurium and Listeria monocytogenes infections. This was explained by a reduction of CD4+ T cell metabolic fitness and cytokine production due to impaired mTOR activity in response to withdrawal of microbial provision of fiber metabolites. Reintroducing dietary fiber efficiently rewired T cell metabolism and restored both mucosal and systemic CD4+ T cell functions and immunity. Finally, dietary intervention study in human volunteers confirmed the impact of short-term dietary switches on human CD4+ T cell functionality. This work reveals that short-term nutritional changes cause a drastic yet transient depression of both mucosal and systemic immunity, creating windows of opportunities for pathogenic infections.
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| Overall design |
RNA sequencing of total tissue from Peyer's patches and Ileum on day 0, 3, 6, 9 and 12 after initial diet change
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| Contributor(s) |
Siracusa F, Schaltenberg N, Kumar Y, Lesker TR, Steglich B, Elinav E, Huber S, Heeren J, Gagliani N |
| Citation(s) |
37580603 |
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| Submission date |
Apr 05, 2023 |
| Last update date |
Oct 10, 2023 |
| Contact name |
Babett Steglich |
| Organization name |
Universitätsklinikum Hamburg-Eppendorf
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| Street address |
Martinistrasse 52
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| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (30)
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| GSM7152331 |
Peyer's Patches, 0 days after first switch, sample #1 |
| GSM7152332 |
Peyer's Patches, 0 days after first switch, sample #2 |
| GSM7152333 |
Peyer's Patches, 0 days after first switch, sample #3 |
| GSM7152334 |
Peyer's Patches, 3 days after first switch, sample #1 |
| GSM7152335 |
Peyer's Patches, 3 days after first switch, sample #2 |
| GSM7152336 |
Peyer's Patches, 3 days after first switch, sample #3 |
| GSM7152337 |
Peyer's Patches, 6 days after first switch, sample #1 |
| GSM7152338 |
Peyer's Patches, 6 days after first switch, sample #2 |
| GSM7152339 |
Peyer's Patches, 6 days after first switch, sample #3 |
| GSM7152340 |
Peyer's Patches, 9 days after first switch, sample #1 |
| GSM7152341 |
Peyer's Patches, 9 days after first switch, sample #2 |
| GSM7152342 |
Peyer's Patches, 9 days after first switch, sample #3 |
| GSM7152343 |
Peyer's Patches, 12 days after first switch, sample #1 |
| GSM7152344 |
Peyer's Patches, 12 days after first switch, sample #2 |
| GSM7152345 |
Peyer's Patches, 12 days after first switch, sample #3 |
| GSM7152346 |
Ileum, 0 days after first switch, sample #1 |
| GSM7152347 |
Ileum, 0 days after first switch, sample #2 |
| GSM7152348 |
Ileum, 0 days after first switch, sample #3 |
| GSM7152349 |
Ileum, 3 days after first switch, sample #1 |
| GSM7152350 |
Ileum, 3 days after first switch, sample #2 |
| GSM7152351 |
Ileum, 3 days after first switch, sample #3 |
| GSM7152352 |
Ileum, 6 days after first switch, sample #1 |
| GSM7152353 |
Ileum, 6 days after first switch, sample #2 |
| GSM7152354 |
Ileum, 6 days after first switch, sample #3 |
| GSM7152355 |
Ileum, 9 days after first switch, sample #1 |
| GSM7152356 |
Ileum, 9 days after first switch, sample #2 |
| GSM7152357 |
Ileum, 9 days after first switch, sample #3 |
| GSM7152358 |
Ileum, 12 days after first switch, sample #1 |
| GSM7152359 |
Ileum, 12 days after first switch, sample #2 |
| GSM7152360 |
Ileum, 12 days after first switch, sample #3 |
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| This SubSeries is part of SuperSeries: |
| GSE229089 |
Synchronized response of CD4+ T cells to short-term dietary changes leads to windows of mucosal and systemic immune depression |
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| Relations |
| BioProject |
PRJNA952796 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE229088_Diet_change_processed.tsv.gz |
1.1 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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