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| Status |
Public on Jul 11, 2023 |
| Title |
Synchronized response of CD4+ T cells to short-term dietary changes - CD4+ T cell RNA sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Omnivorous animals, including mice and humans, tend to prefer energy-dense nutrients rich in fat over plant-based diets, especially for short periods of time. The health consequences of this short-term consumption of energy-dense nutrients remain still unclear. We found that every short-term, reiterated switches to feast diets mimicking our social eating behavior, breached the potential buffering effect of the intestinal microbiota and deeply reorganized the immunological architecture of mucosa-associated lymphoid tissues. The first dietary switch was sufficient to induce transient mucosal immune depression and suppress systemic, antigen-specific immunity leading to higher susceptibility to Salmonella Typhimurium and Listeria monocytogenes infections. This was explained by a reduction of CD4+ T cell metabolic fitness and cytokine production due to impaired mTOR activity in response to withdrawal of microbial provision of fiber metabolites. Reintroducing dietary fiber efficiently rewired T cell metabolism and restored both mucosal and systemic CD4+ T cell functions and immunity. Finally, dietary intervention study in human volunteers confirmed the impact of short-term dietary switches on human CD4+ T cell functionality. This work reveals that short-term nutritional changes cause a drastic yet transient depression of both mucosal and systemic immunity, creating windows of opportunities for pathogenic infections.
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| Overall design |
RNA sequencing of FACS sorted CD4+ T cells from Peyer's patches and draining lymph nodes after OVA challenge
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| Contributor(s) |
Siracusa F, Schaltenberg N, Kumar Y, Lesker TR, Steglich B, Elinav E, Huber S, Heeren J, Gagliani N |
| Citation(s) |
37580603 |
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| Submission date |
Apr 05, 2023 |
| Last update date |
Oct 10, 2023 |
| Contact name |
Babett Steglich |
| Organization name |
Universitätsklinikum Hamburg-Eppendorf
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| Street address |
Martinistrasse 52
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| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| GSM7152322 |
CD45.1+ OT-II viable T cells from dLN, regular diet #1 |
| GSM7152323 |
CD45.1+ OT-II viable T cells from dLN, regular diet #2 |
| GSM7152324 |
CD45.1+ OT-II viable T cells from dLN, feast diet #1 |
| GSM7152325 |
CD45.1+ OT-II viable T cells from dLN, feast diet #2 |
| GSM7152326 |
FACS-sorted CD4+Foxp3- T cells from Peyer's Patches, regular diet #1 |
| GSM7152327 |
FACS-sorted CD4+Foxp3- T cells from Peyer's Patches, regular diet #2 |
| GSM7152328 |
FACS-sorted CD4+Foxp3- T cells from Peyer's Patches, regular diet #3 |
| GSM7152329 |
FACS-sorted CD4+Foxp3- T cells from Peyer's Patches, feast diet #1 |
| GSM7152330 |
FACS-sorted CD4+Foxp3- T cells from Peyer's Patches, feast diet #2 |
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| This SubSeries is part of SuperSeries: |
| GSE229089 |
Synchronized response of CD4+ T cells to short-term dietary changes leads to windows of mucosal and systemic immune depression |
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| Relations |
| BioProject |
PRJNA952795 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE229087_CD4_processed.tsv.gz |
468.1 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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