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Series GSE229062 Query DataSets for GSE229062
Status Public on Dec 06, 2023
Title Marker-based CRISPR screening reveals a direct MED12-p63 interaction that activates basal identity in pancreatic ductal adenocarcinoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We report a novel p63-MED12 transcriptional complex that is selectively required for basal pancreatic cancer and squamous cell carcinoma survival. This transcription factor-imposed genetic vulnerability on a general coactivator will inform future p63-blocking therapeutic strategies.
Overall design RNA-seq and ChIP-seq of p63 and MED12 manipulation in basal and classical human pancreatic cancer cell lines. FACS-based and negative selection CRISPR screens (genome-wide, Mediator exon tiling) to nominate lineage regulators of basal pancreatic cancer.
Contributor(s) Maia-Silva D, Vakoc CR
Citation(s) 37961243
Submission date Apr 05, 2023
Last update date Dec 20, 2023
Contact name Diogo Maia e Silva
Organization name Cold Spring Harbor Laboratory
Street address 1 Bungtown Road
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (276)
GSM7151301 D3 KLM1-sgCDK1-6e1-rep1
GSM7151302 D3 KLM1-sgCDK1-6e1-rep2
GSM7151303 D3 KLM1-sgCDK1-6e1-rep3
BioProject PRJNA952663

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE229062_BxPC3.h5Seurat 386.2 Mb (ftp)(http) H5SEURAT
GSE229062_RAW.tar 11.0 Gb (http)(custom) TAR (of BW, TXT)
GSE229062_T3M4.h5Seurat 479.6 Mb (ftp)(http) H5SEURAT
GSE229062_hF3.h5Seurat 1.2 Gb (ftp)(http) H5SEURAT
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Raw data are available in SRA
Processed data provided as supplementary file

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