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| Status |
Public on Jan 01, 2024 |
| Title |
Single-cell RNA-sequencing of osteocyte-derived mitochondria recipient cells in the intra-tibial injection of LLC1 cells murine model. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
LLC1 cells were injected into the tibia of DMP1-Cre PhAM mice to study the intercellular mitochondrial transfer in the bone microenvironment. We used single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity of osteocyte-derived mitochondria recipient cells.
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| Overall design |
LLC1 cells were injected intra-tibially into DMP1-Cre PhAM mice. 14 days later tumor-bearing tibia were dissected and enzymatically dissociated into single cell suspension. Osteocyte-derived mitochondria recipient cells were isolated by fluorescence-activated cell sorting (FACS) according to the presence of Dendra2 signal and analyzed using scRNAseq.
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| Contributor(s) |
Zhou H, Zhang W, Gao J, Ye Z |
| Citation(s) |
38232158 |
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| Submission date |
Mar 30, 2023 |
| Last update date |
Jan 24, 2024 |
| Contact name |
Zhaoming Ye |
| E-mail(s) |
yezhaoming@zju.edu.cn
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| Organization name |
The Second Affiliated Hospital, Zhejiang University School of Medicine
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| Street address |
88 jiefang road
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| City |
hangzhou |
| ZIP/Postal code |
310031 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
| GSM7133971 |
mitochondria-recipient cells in intratibial LLC model |
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| Relations |
| BioProject |
PRJNA950443 |
