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| Status |
Public on Sep 01, 2023 |
| Title |
Transcriptome analysis of avocado embryogenic cultures selected for resistance to Rosellinia necatrix exudates |
| Organism |
Persea americana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Embryogenic cultures derived from a zygotic embryo of the avocado cv. Anaheim, were selected for resistance to the culture filtrate (CF) of Rosellinia necatrix, the causal agent of avocado white root rot. Cultures were obtained through recurrent selections in progressively increasing concentrations of fungal CF (from 20% up to 80%).
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| Overall design |
RNA-Seq technology was used to compare the transcriptomic profiles of the avocado callus tolerant line L3 (capable to survive in the presence of 80% CF) and control line AN-9 (not exposed to CF), after 24 hours of growth in a medium containing 40% CF.
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| Contributor(s) |
Moreno-Pérez A, Zumaquero A, Martínez-Ferri E, Barceló-Muñoz A, Lopez-Herrera C, Pliego-Alfaro F, Palomo-Ríos E, Pliego C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 27, 2023 |
| Last update date |
Sep 01, 2023 |
| Contact name |
Clara Pliego |
| E-mail(s) |
mclara.pliego@juntadeandalucia.es
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| Phone |
0034654564365
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| Organization name |
IFAPA
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| Department |
Department of Genomics and Biotechnology
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| Lab |
Biotechnology
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| Street address |
Cortijo de la Cruz
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| City |
Malaga |
| State/province |
Malaga |
| ZIP/Postal code |
29140 |
| Country |
Spain |
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| Platforms (1) |
| GPL33284 |
Illumina NovaSeq 6000 (Persea americana) |
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| Samples (12)
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| GSM7118443 |
Tolerant callus line L3, exposed to fungal filtrate TCLF1 |
| GSM7118444 |
Tolerant callus line L3, exposed to fungal filtrate TCLF2 |
| GSM7118445 |
Tolerant callus line L3, exposed to fungal filtrate TCLF3 |
| GSM7118446 |
Tolerant callus line L3, non exposed to fungal filtrate TCL1 |
| GSM7118447 |
Tolerant callus line L3, non exposed to fungal filtrate TCL2 |
| GSM7118448 |
Tolerant callus line L3, non exposed to fungal filtrate TCL3 |
| GSM7118449 |
Susceptible callus line AN-9, exposed to fungal filtrate SCLF1 |
| GSM7118450 |
Susceptible callus line AN-9, exposed to fungal filtrate SCLF2 |
| GSM7118451 |
Susceptible callus line AN-9, exposed to fungal filtrate SCLF3 |
| GSM7118452 |
Susceptible callus line AN-9, non exposed to fungal filtrate SCL1 |
| GSM7118453 |
Susceptible callus line AN-9, non exposed to fungal filtrate SCL2 |
| GSM7118454 |
Susceptible callus line AN-9, non exposed to fungal filtrate SCL3 |
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| Relations |
| BioProject |
PRJNA949214 |
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