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Series GSE227938 Query DataSets for GSE227938
Status Public on Apr 19, 2023
Title Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation [EPS_CL]
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary We have used a combined transcriptome-proteome approach to describe how EPS affected the cargo of extracellular vesicles derived from myotubes from morbidly obese patients with T2D, and revealed several new factors, both miRs and proteins, that might act as exercise factors.
During exercise, skeletal muscles release signaling factors that communicate with other organs and mediate beneficial effects of exercise. These factors include myokines, metabolites, and extracellular vesicles (EVs). In the present study, we have examined how electrical pulse stimulation (EPS) of myotubes, a model of exercise, affects the cargo of released EVs. Chronic low frequency EPS was applied for 24 h to human myotubes isolated and differentiated from biopsy samples from 6 morbidly obese females with T2D, and EVs, both exosomes and microvesicles (MV), were isolated from cell media 24 h thereafter.Protein content was assessed by high-resolution proteomic analysis (LC-MS/MS), non-coding RNA was quantified by Affymetrix microarray GeneChip TM Multispecies miRNA 4.0 and Flash Tag TM Biotin RNA labelling, Thermo Fisher), and selected microRNAs (miRs) validated by real time RT-qPCR. We found that human myotubes secrete both exosomes and MV. EPS treatment of the myotubes, clearly changed the protein content of both exosomes and MV, whereas the miR content was changed only in exosomes. We suggest that skeletal muscle derived EVs can contribute to circulatory EVs and mediate beneficial effects of exercise in metabolically active organs.
Overall design Number of patients: 4. Each patient was their own control by comparing the RNA and protein-cargo before and after EPS. Cellular activation by EPS was detected by IL-6 analysis. Non template controls and spike ins control probe sets were included in the microarray analysis. The Affymetrix GeneChip Command Console sodtware was used for normalization, the Robust Multichip Analysis (RMA) alogoritm was applied for signal generation, and Partek R Genomics SuiteTM software for statistical analyis. Validation of microarray results was carried out by RT-qPCR of selected miRNAs.
Contributor(s) Aas V, Øvstebø R, Brusletto BS, Aspelin T, Trøseid A, Qureshi S, Eid DS, Olstad OK, Nyman T, Haug KF
Citation(s) 37064893
Submission date Mar 22, 2023
Last update date Apr 21, 2023
Contact name Kari Bente Foss Haug
Phone 93047410
Organization name Oslo University Hospital
Department Medical Biochemistry
Street address Skogstien 28
City Blommenholm
ZIP/Postal code 1365
Country Norway
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (8)
GSM7111196 CL14-EPS
GSM7111197 CL14+EPS
GSM7111198 CL47-EPS
This SubSeries is part of SuperSeries:
GSE227939 Distinct microRNA and protein profiles of extracellular vesicles secreted from myotubes from morbidly obese donors with type 2 diabetes in response to electrical pulse stimulation
BioProject PRJNA947541

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE227938_RAW.tar 5.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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