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Series GSE227370 Query DataSets for GSE227370
Status Public on Jan 15, 2024
Title Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation (Figure2)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Long-lived plasma cells (PCs) secrete antibodies that can provide sustained immunity against infection. It has been proposed that high affinity cells are preferentially selected into this compartment, potentiating the immune response. We used single cell RNA-seq to track the germinal center (GC) development of Ighg2A10 cells, specific for the Plasmodium falciparum circumsporozoite protein (PfCSP). Following immunization with Plasmodium sporozoites we identified 3 populations of cells in the GC light zone. One population expressed a gene signature associated with the initiation of PC differentiation and had an enhanced propensity to form PCs in vitro. Unexpectedly, the estimated affinity of this putative pre-PC population was indistinguishable from cells in the GC generally. This was also true when high- or low-avidity recombinant PfCSP proteins were used as immunogens. Immunization with low-avidity PfCSP did, however, induce increased affinity maturation. Collectively these findings suggest that the initiation of PC development in the GC occurs via an affinity independent process.
Overall design Probe+ IgD- Ighg2A10 B cells stained with TotalSeq-C hashtag antibodies were isolated by Fluorescence-activated cell sorting (FACS) from recipient mice that had been immunized with irradiated sporozoites either 7 or 21 days prior. The transcriptome and VDJ sequences of sorted cells was then analyzed using scRNAseq.
Contributor(s) Sutton HJ, Cockburn I
Citation(s) 38228150
Submission date Mar 14, 2023
Last update date Apr 15, 2024
Contact name Xin Gao
Phone 0450031169
Organization name The Australian National University
Department Immunology
Street address 131 Garran Rd
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM7099100 Sample1_D7_Antibody_Capture
GSM7099101 Sample1_D7_Transcriptome
GSM7099102 Sample1_D7_VDJ
This SubSeries is part of SuperSeries:
GSE248377 Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation
BioProject PRJNA944714

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Supplementary file Size Download File type/resource
GSE227370_RAW.tar 241.4 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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