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Series GSE227110 Query DataSets for GSE227110
Status Public on Aug 25, 2023
Title The effect of a BCCA-free diet on 1W pressure overload-induced cardiac gene expression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Identifying branched-chain amino acid (BCAA) oxidation enzymes in the nucleus led us to predict that they are a source of propionyl-CoA that could be utilized for histone propionylation and, thereby, regulate gene expression. To investigate this and its effect on the development of cardiac hypertrophy and failure, we applied pressure overload on the heart in mice maintained on a diet with standard levels of BCAA (BCAA-control) versus a BCAA-free diet. The former was associated with an increase in H3K23-propionyl (H3K23Pr) at the promoters of upregulated genes [e.g., cell signaling and extracellular matrix (ECM) genes] and a decrease at the promoters of downregulated genes [e.g., electron transfer complex (ETC I-V) and metabolic genes]. Intriguingly, the BCAA-free diet diminished the increase in promoter-H3K23Pr and significantly reduced ECM gene expression and collagen deposition. Conversely, the BCAA-free diet abolished the downregulation of ETC I-V subunits, enhanced mitochondrial respiration, and curbed cardiac hypertrophy. Thus, lowering the intake of BCAA reduces histone propionylation, which moderates the effects of stress on the heart.
 
Overall design Mice were fed a BCAA-free diet or a BCAA-control diet for 4 days. Mice were then subjected to a sham or transverse aortic constriction (TAC) surgery for 1-week (1W), while being maintained on the same diets. The heart function and structure was asssessed by echocardiography, the heart isolated, RNA extracted and analyzed by RNA-Seq (Azenta).
 
Contributor(s) Maha A
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Submission date Mar 10, 2023
Last update date Aug 25, 2023
Contact name Maha Abdellatif
E-mail(s) abdellma@rutgers.edu
Phone 9739721254
Organization name Rutgers University
Department Cell Biology and Molecular Medicine
Street address 185 S. Orange Ave., MSB
City Newark
State/province NJ
ZIP/Postal code 07103
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (12)
GSM7091773 BCAA-control, sham, rep1 [BCS1]
GSM7091774 BCAA-control, sham, rep2 [BCS2]
GSM7091775 BCAA-control, sham, rep3 [BCS3]
This SubSeries is part of SuperSeries:
GSE229131 Lowering dietary BCAA reduces fibrosis, enhances mitochondrial respiration, and curbs cardiac hypertrophy, via regulating histone H3K23-propionylation
Relations
BioProject PRJNA943243

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE227110_BCT-vs-BCS.csv.gz 1.4 Mb (ftp)(http) CSV
GSE227110_BFS-vs-BCS.csv.gz 1.4 Mb (ftp)(http) CSV
GSE227110_BFT-vs-BCT.csv.gz 1.4 Mb (ftp)(http) CSV
GSE227110_BFT-vs-BFS.csv.gz 1.3 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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