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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 20, 2023 |
| Title |
Nrf2 drives hepatocellular carcinoma progression through acetyl-CoA-mediated metabolic, epigenetic and oncogenic regulatory networks |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Liver tumors had high levels of histone acetylation. Nrf2 knockout mice developed fewer tumors than Nrf2 wild-type mice. The mechanistic study found that Nrf2 knockout reduced the generation of acetyl CoA from impaired glycolysis, TCA cycle, and fatty acid metabolism. Acetyl CoA is the substrate for protein acetylation including histone acetylation. Here we determined the genome-wide distribution of AcH3K27. We found that Nrf2 through regulating acetyl CoA production affects histone acetylation (AcH3K27) to modulate the expression of genes, whose products were involved in the glycolysis, TCA cycle, fatty acid metabolism, and oncogenic Myc/mTor signaling. Our findings supported an Nrf2-integrated metabolic, epigenetic and oncogenic signaling in driving liver tumor development.
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| Overall design |
Comparison the genome-wide distribution of histone lysine 27 acetylation in liver tumors of Nrf2 WT (2 samples) and Nrf2 KO mice (2 samples) at 36 weeks of age.
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| Contributor(s) |
Xi C, Pang J, Zhu X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 10, 2023 |
| Last update date |
Mar 22, 2023 |
| Contact name |
Xingguo Zhu |
| E-mail(s) |
zhughsu@gmail.com
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| Phone |
7067216301
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| Organization name |
Augusta University
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| Department |
Cancer Center
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| Street address |
1120 15th st, CN4114, CN4114
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| City |
Augusta |
| State/province |
Georgia |
| ZIP/Postal code |
30912 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| GSM7091014 |
Mouse Liver tumor, Nrf2 WT, AcH3K27, rep1 |
| GSM7091015 |
Mouse Liver tumor, Nrf2 WT, AcH3K27, rep2 |
| GSM7091016 |
Mouse Liver tumor, Nrf2 KO, AcH3K27, rep1 |
| GSM7091017 |
Mouse Liver tumor, Nrf2 KO, AcH3K27, rep2 |
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| Relations |
| BioProject |
PRJNA943154 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE227068_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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