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Series GSE22690 Query DataSets for GSE22690
Status Public on Jul 14, 2010
Title The intracellular domain of amyloid precursor protein induces the dynamic change of the gene expression profile in P19 cells.
Organism Mus musculus
Experiment type Expression profiling by array
Summary As previously reported (Neuroscience letters 444(2008)127-131), we established embryonic carcinoma P19 cell line expressing the intracellular domain of APP (AICD). Although neurons could be differentiated from these cell lines with RA treatment, expression of AICD gave rise to neuron-specific apoptosis. To identify the genes that are involved in this cell death, we evaluated changes of gene expressions that was induced by AICD through this process of cell death. The expression profiles of almost forty thousands transcripts were monitored by DNA microarrays.
Overall design AICD-expressing P19 cell lines (AICD/P19) and control P19 cell lines (pCDNA/P19) that carry vector alone were used and cultured. To induce neural differentiation of these P19 cell lines, cells were allowed to aggregate in culture medium with 5×10-7 M all-trans-retinoic acid (RA) for 4 days (aggregated state). After aggregation, cells were replated onto cell culture grade dishes or poly-L-Lysine coated cell-disks for two days (differentiated state). RNAs were isolated at each states.
Contributor(s) Nakayama K, Ohkawara T
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Submission date Jul 02, 2010
Last update date Jan 12, 2017
Contact name Kohzo Nakayama
E-mail(s) kohzona@
Phone +81-263-37-2594
Fax +81-263-37-2594
Organization name Shinshu University
Department Anatomy
Street address Asahi
City Matsumoto
ZIP/Postal code 390-8621
Country Japan
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (6)
GSM562353 pCDNA/P19_undifferentiated
GSM562354 AICD/P19_undifferentiated
GSM562355 pCDNA/P19_aggregated
BioProject PRJNA128295

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22690_RAW.tar 11.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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