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| Status |
Public on Mar 09, 2023 |
| Title |
B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection [single cell RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Germinal centers (GCs) are sites of B cell clonal expansion, diversification, and antibody affinity selection. This process is limited and directed by T follicular helper cells that provide helper signals to B cells that endocytose, process, and present cognate antigens in proportion to their B cell receptor (BCR) affinity. Under this model, the BCR functions as an endocytic receptor for antigen capture. How signaling through the BCR contributes to selection is not well understood. To investigate the role of BCR signaling in GC selection, we developed a tracker for antigen binding and presentation and a Bruton’s tyrosine kinase drug-resistant-mutant mouse model. We showed that BCR signaling per se is necessary for the survival and priming of light zone B cells to receive T cell help. Our findings provide insight into how high-affinity antibodies are selected within GCs and are fundamental to our understanding of adaptive immunity and vaccine development.
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| Overall design |
LZ B1-8 BTKC481S and LZ B18 BTKWT cells were sorted from acalabrutinib or vehicle treated hosts.
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| Contributor(s) |
Chen ST, Oliveira TY, Gazumyan A, Cipolla M, Nussenzweig MC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 18, 2023 |
| Last update date |
Mar 09, 2023 |
| Contact name |
Thiago Y Oliveira |
| E-mail(s) |
toliveira@rockefeller.edu
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| Organization name |
The Rockefeller University
|
| Department |
Immunology, Virology and Microbiology
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| Lab |
Laboratory of Molecular Immunology
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| Street address |
1230 YORK AVE
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| City |
NEW YORK |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE225575 |
B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection |
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| Relations |
| BioProject |
PRJNA936433 |
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