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Series GSE225317 Query DataSets for GSE225317
Status Public on Apr 24, 2024
Title Trophectoderm biopsies of blastocysts for preimplantation genetic testing following in vitro fertilization and embryo culture increases epigenetic dysregulation in a mouse model [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Trophectoderm biopsy for preimplantation genetic testing (TEBx) impacts placental and embryonic health during early development, with some alterations resolving while others worsening later in development. We observed that at E12.5, IVF + TEBx had a worse outcome in terms of changes in DNA methylation and differential gene expression in placentas and whole embryos compared with IVF alone. These changes were reflected in alterations in placental morphology and blood vessel density. At E18.5, early changes in embryos were maintained or worsened, while molecular and morphological changes in the placenta were not different from the IVF group, except for changes in blood vessel density, which persisted. Of note is that most differences were sex specific. We conclude that TEBx has more detrimental effects in both mid-gestation placental and embryonic tissues, with changes in embryonic tissues persisting or worsening in later developmental stages compared to IVF alone. Additionally, consistent with previous work, we report sex differences in the observed effects. Finally, we identified that the addition of vitrification after TEBx has a more pronounced detrimental effect on placental and fetal health. Our findings provide additional support that more studies should be done to assess the impact of new procedures during ART to ensure healthy pregnancies and offspring outcomes.
 
Overall design Gene expression analysis using Total RNA extracted from E12.5 placenta n=30 (F=15 and M=15), E12.5 whole fetus n=30 (F=15 and M=15), E18.5 placentas (F=15 and M=15) and E18.5 fetal livers (F=15 and M=15) from 3 different groups Natural, IVF and IVF+TEBx were sequenced using Illumina NovaSeq6000
 
Contributor(s) Rhon-Calderon EA, Vrooman LA, Rosier CL, Ord T, Mainigi M, Schultz RM, Bartolomei MS
Citation(s) 37994669
Submission date Feb 14, 2023
Last update date Apr 24, 2024
Contact name Eric Alejandro Rhon Calderon
E-mail(s) eric.rhoncalderon@pennmedicine.upenn.edu
Phone 2152909019
Organization name University of Pennsylvania
Department Cell and Developmental Biology
Lab Bartolomei Lab
Street address 3400 Civic Center Blvd, Bldg 421, Floor 9-175
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (110)
GSM7044156 RNA library from E12.5 Female IVF Placenta 1
GSM7044157 RNA library from E12.5 Female IVF Placenta 2
GSM7044158 RNA library from E12.5 Female IVF Placenta 3
This SubSeries is part of SuperSeries:
GSE225318 Trophectoderm biopsies of blastocysts for preimplantation genetic testing following in vitro fertilization and embryo culture increases epigenetic dysregulation in a mouse model
Relations
BioProject PRJNA934960

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Supplementary file Size Download File type/resource
GSE225317_E125_Placentas_DEGs.xlsx 17.2 Mb (ftp)(http) XLSX
GSE225317_E125_wholefetus_DEGs.xlsx 16.1 Mb (ftp)(http) XLSX
GSE225317_E185_Placentas_DEGs.xlsx 16.5 Mb (ftp)(http) XLSX
GSE225317_E185_fetalliver_DEGs.xlsx 16.2 Mb (ftp)(http) XLSX
GSE225317_summary_count_E12.5_Placenta.txt.gz 1.0 Mb (ftp)(http) TXT
GSE225317_summary_count_E12.5_WholeFetus.txt.gz 1.1 Mb (ftp)(http) TXT
GSE225317_summary_count_E18.5_FetalLiver.txt.gz 773.6 Kb (ftp)(http) TXT
GSE225317_summary_count_E18.5_Placenta.txt.gz 1.0 Mb (ftp)(http) TXT
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