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Series GSE225308 Query DataSets for GSE225308
Status Public on Dec 06, 2023
Title Mature oligodendrocyte (OL) translatome changes at various times after moderate contusive spinal cord injury at the thoracic T9 level
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary OL translatome was determined in female RiboTag:Plp1-CreERT2 mice (C57Bl6 background) at 2, 10, and, 42 days after moderate contusive T9 SCI. Ribotag expression was induced by daily tamoxifen injections (1 mg i.p. over 8 days). Three weeks after completion of the induction protocol moderate contusive SCI was performed at T9 level (IH imactor, 50 kdyn). Uninjured mice (naive) were used as controls. At the time of surgery, animal age was 10-12 weeks. After euthanasia, RNA was isolated from a 5 mm spinal cord segment spanning the injury epicenter. OL tranlsatome was immunopurified using anti-HA antibody and protein A/G coupled magnetic beads following the Ribotag protocol. Total spinal cord RNA and OL translatome RNA samples were analyzed by RNASeq. Each biological sample was prepared by pooling material from two mice (3 biological samples representing 6 animals were used for each timepoint).
Overall design Analyzing effects of spinal cord injury on mRNA levels in total spinal cord transcriptome and OL translatome
Contributor(s) Forston M, Wei G, Hetman M, Whittemore S, Chariker JH, Rouchka EC
Citation(s) 38040794
NIH grant(s)
Grant ID Grant title Affiliation Name
P20 GM103436 IDeA Networks of Biomedical Research Excellence in Kentucky UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION MARTHA E BICKFORD
Submission date Feb 14, 2023
Last update date Dec 06, 2023
Contact name Eric Christian Rouchka
Organization name University of Louisville
Department Biochemistry and Molecular Genetics
Lab KY INBRE Bioinformatics Core
Street address 522 East Gray Street
City Louisville
State/province Kentucky
ZIP/Postal code 40292
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (24)
GSM7043880 RNASeq, control-IN, biological sample 1
GSM7043881 RNASeq, control-IP, biological sample 1
GSM7043882 RNASeq, control-IN, biological sample 2
BioProject PRJNA934931

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Supplementary file Size Download File type/resource
GSE225308_GeneMatrix_RawCounts.txt.gz 748.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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