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Series GSE225305 Query DataSets for GSE225305
Status Public on Jul 19, 2023
Title An engineered Sox17 induces somatic to neural stem cell fate transition independently from pluripotency reprogramming [RRBS]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Advances in molecular strategies to reprogram cell fate is a promising avenue for cell-based therapies. Methods to generate induced neural stem cells (iNSCs) are often slow with limited reprogramming events and it is unclear whether cells transit via a pluripotent state. We report an iNSC reprogramming approach from embryonic and aged mouse fibroblasts using an engineered Sox17 (eSox17FNV). eSox17FNV efficiently drives iNSC reprogramming while Sox2 or Sox17 fails to do so. eSox17FNV acquires the capacity to bind new protein partners to scan the genome more efficiently and possesses a more potent transactivation domain than Sox2. At the onset of reprogramming, in the presence of eSox17FNV, fibroblasts divert from a iPSC route towards iNSC fate. Further, lineage tracing shows that emerging iNSCs do not transit through a pluripotent state if POU factors are excluded. This reveals new molecular and physiological framework for iNSC generation and contrasts with lineage from pluripotency reprogramming.
 
Overall design We used reduced representation bisulfite sequencing (RRBS) to analyze the genomic DNA methylation of young and old primary NSCs and fibroblasts and mouse iNSCs generated from old fibroblasts.
 
Contributor(s) Weng M, Jauch R
Citation(s) 37611093
Submission date Feb 14, 2023
Last update date Sep 14, 2023
Contact name Mingxi WENG
E-mail(s) mingxi@connect.hku.hk
Phone 55326522
Organization name School of Biomedical Sciences, The University of Hong Kong
Street address L408A, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong
City Hong Kong
State/province Not Applicable
ZIP/Postal code 000000
Country Hong Kong
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (13)
GSM7043846 FS2GNSCR1
GSM7043847 FS2GNSCR2
GSM7043848 ICRMEFR1
This SubSeries is part of SuperSeries:
GSE195556 An engineered Sox17 induces somatic to neural stem cell fate transition independently from pluripotency reprogramming
Relations
BioProject PRJNA934927

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE225305_RAW.tar 323.3 Mb (http)(custom) TAR (of COV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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