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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 01, 2023 |
| Title |
A monoclonal Trd chain supports the development of the complete set of functional γδ T cell lineages (NGS) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The clonal selection theory describes the key features of adaptive immune responses of B and T cells. For αβ T cells and B cells antigen recognition and selection principles are known at a detailed molecular level. The precise role of the antigen receptor in γδ T cells remains less well understood. To better understand the role of the γδ T cell receptor (TCR), in particular its specificity for thymic selection of γδ T cells and for γδ T cell biology in general, we generated a novel orthotopic TCRδ transgenic mouse model. We demonstrate a multi-layered functionality of γδ TCRs and diverse roles of CDR3δ-mediated selection during γδ T cell development. Whereas epithelial populations using Vγ5 or Vγ7 chains are affected to only minor extend in their biology in the presence of a single TCRδ chain, pairing with Vγ1 positively selects subpopulations of γδ T cells with distinct programs in several organs, thereby distorting the repertoire. In conclusion, our data support dictation of developmental tropism together with adaptive-like recognition principles in a single antigen receptor.
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| Overall design |
Murine γδ T cells were isolated from the spleen via Fluorescence-activated cell sorting (FACS) according to the presence of CD3 and γδTCR and the absence of αβTCR, B220 and CD11c, and analyzed using scRNAseq for transcriptome and V(D)J repertoire analysis.
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| Contributor(s) |
Hahn AM, Vogg L, Brey S, Schneider A, Schäfer S, Palmisano R, Pavlova A, Sandrock I, Tan L, Fichtner AS, Prinz I, Ravens S, Winkler TH |
| Citation(s) |
37182210 |
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| Submission date |
Feb 10, 2023 |
| Last update date |
Jun 01, 2023 |
| Contact name |
Simon Schaefer |
| E-mail(s) |
anon1235813213455@gmail.com
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| Phone |
017670775922
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| Organization name |
FAU Erlangen
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| Department |
Genetics
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| Lab |
Thomas Winkler
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| Street address |
Glueckstr. 6
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| City |
Erlangen |
| State/province |
Bavaria |
| ZIP/Postal code |
91054 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (12)
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| GSM7037561 |
TRD_TRG, spleen, WT, replicate 2 |
| GSM7037562 |
TRD_TRG, spleen, KI, replicate 3 |
| GSM7037563 |
TRD_TRG, spleen, WT, replicate 3 |
| GSM7037564 |
TRD_TRG, SI-IEL, KI, replicate 1 |
| GSM7037565 |
TRD_TRG, SI-IEL, WT, replicate 1 |
| GSM7037566 |
TRD_TRG, SI-IEL, KI, replicate 2 |
| GSM7037567 |
TRD_TRG, SI-IEL, WT, replicate 2 |
| GSM7037568 |
TRD_TRG, SI-IEL, KI, replicate 3 |
| GSM7037569 |
TRD_TRG, SI-IEL, WT, replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE225036 |
A monoclonal Trd chain supports the development of the complete set of functional γδ T cell lineages |
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| Relations |
| BioProject |
PRJNA934584 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE225034_RAW.tar |
56.6 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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