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| Status |
Public on May 09, 2023 |
| Title |
Tumor-intrinsic ST3GAL3 Activity Blunts Anti-tumor Immune and Drives Immunotherapy Resistance in Ovarian Cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
ST3GAL3 is a α2,3-sialyltransferase and involves in α2,3 sialylation in tumors; however, its role in the tumorigenic capacity of malignant cells and anti-tumor immune remains largely unexplored. Here, HM1 cells were stably transfected using lentivirus expressing a shRNA targeting ST3GAL3 (HM1-shST3GAL3) or scramble shRNA as control (HM1-shControl). Then, HM1-shControl or HM1-shST3GAL3 cells were injected into the right flank of B6C3F1 mice using a 27 g needle. ST3GAL3 knockdown significantly compromised tumor growth at a immune-dependant manner. Mechanistically, ST3GAL3 knockdown enhances pro-inflammatory phenotype of macrophages, leading ultimately to activation and recruitmentof CD8+ T cells. Moreover, we identified ST3GAL3 as a potential novel biomarker for bevacizumab or immune checkpoint blockade (ICB) intervention. ST3GAL3 knockdown in tumors also be useful to improve cancer immunotherapies.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for shControl tumor tissues (shControl tumors, n = 3) and shST3GAL3 tumor tissues (shST3GAL3 tumors, n = 3) of early tumor model.
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| Contributor(s) |
Liu H, Cao K |
| Citation(s) |
37172314 |
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| Submission date |
Feb 08, 2023 |
| Last update date |
Aug 11, 2023 |
| Contact name |
Haiou Liu |
| E-mail(s) |
liuhaiou@fudan.edu.cn
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| Phone |
+8613916273769
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| Organization name |
Fudan University
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| Street address |
No.128, Shengyang road, Yangpu distinct, Shanghai, P. R. China.
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| City |
Shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA932806 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE224856_RAW.tar |
7.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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